BACKGROUND:MicroRNAs (miRNAs) are small, non-coding RNAs that are important for post-transcriptional gene regulation in both healthy and morbid conditions. Numerous miRNAs promote tumorigenesis, while others have a tumour suppressive effects. Acute myeloid leukaemia (AML) is a heterogeneous group of genetically diverse hematopoietic malignancies with variable response to treatment.AIM:Our study aimed to investigate the possible role of miR-150 in de novo adult AML and the impact of its level on survival, and we used in the silicon analysis to predict the main target genes involved in miR-150 mediated cancer pathway.MATERIAL AND METHODS:We evaluated miR-150 expression profiling assay using TaqMan primer probes RT-PCR in the plasma of 50 adult AML patients, before the start of treatment and at day 28 of treatment, along with 20 normal adult control samples. miR-16 was used as an endogenous reference for standardisation. Follow-up of patients during treatment at day 28 of induction chemotherapy and after one year was done.RESULTS:In this study, we found a significantly lower level of miR-150 in AML patients when compared to controls (p = 0.005) with 0.62 fold change than in healthy controls. Patients were divided into two groups: the low miR-150 group (miR-150 < 1) and the high miR-150 group (miR-150 > 1). A statistically significant difference was found between the two groups regarding initial total leukocytic count and initial PB blast count while for the TLC, HB and PLT count at follow up. No difference in the overall survival between the low and the high miR-150 groups could be demonstrated.CONCLUSION:Our results suggest that miR-150 functions as a tumour suppressor and gatekeeper in inhibiting cell transformation and that its downregulation is required for leukemogenesis.

SUMMARYAcute myeloid leukemia (AML) in children with certain genetic aberrations is still associated with inferior outcome. One of these AML subtypes has a translocation t(7;12)(q36;p13) and in this study we have investigated the transforming event behind this AML and possible ways to target its effects. This translocation leads to a gene fusion MNX1::ETV6 but also to high ectopic expression of MNX1. However, only MNX1 was able to induce AML in a mouse model and this was only observed using hematopoietic stem and progenitor cells (HSPC) derived from fetal origin and not from adult bone marrow. This AML was highly penetrant in immunocompromised mice but only partly so in immunocompetent mice. The restriction in transforming capacity to cells from fetal liver origin is in concordance with the clinical finding that t(7;12)(q36;p13) AML is mostly restricted to infants. Molecularly, ectopic expression of MNX1 led to increased H3K4mono, di- and trimethylation and reduction in H3K27me3, both globally and on specific gene promoters. These alternations in histone methylation appeared to be mediated through MNX1 interaction with the methionine cycle and different methyl transferases. The alternations in histone methylation were accompanied with changes in genome wide chromatin accessibility as evident by ATAC-sequencing. We also found that high MNX1 expression resulted in increased DNA damage, which was accompanied by a depletion in the Lin-/Sca1+/c-Kit+ population and skewing toward myeloid lineage. These effects could be prevented by Sinefungin treatment of HSPC, which also halted leukemia development in mice. Comparison of gene expression profile of leukemia cells from our mouse AML model with human induced pluripotent stem cells with t(7;12) differentiated into HSPC showed similar pathway enrichment. In conclusion, we have shown the importance of MNX1 in leukemia development in the t(7;12) AML subtype and the rationale for targeting MNX1 and its downstream pathways.

Schwannoma is a usually benign peripheral nerve sheath tumor with rare breast incidence and few reported cases. We report a case which was presented to and managed in the Oncology center, Mansoura University in November 2017.

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Mobile and wireless devices become widespread devices in the past few years, These devices made substantial progress in the important field of wireless applications which used in all areas of human life. This research develops two applications which execute on mobile and computer. The first application is electronic library which make the user search in the library electronically on computer throw Bluetooth wireless technology, The second application is mouse controlling throw Bluetooth.These applications are programmed with J2ME language on mobiles, covered MIDP 2.0 with CLDC 1.0 executed on mobiles with versions Nokia 6600, Nokia 7610, N72 and executed on computers with the following operating systems Windows 7, Windows XP and Linux Mandriva 2010 .