In recent years, evaluation the effects of alkaloids extraction on the environment is considered regarding the importance of environmental issues. Tropane alkaloids such as hyoscyamine and scopolamine have many applications in the medical field. The purpose of the present study is using the Life Cycle Assessment (LCA) in the environmental impact assessment of total alkaloid extracted from Atropa belladonna, so all the usable materials and technologies as well as the diffusion of various substances into the environment were estimated for 6.2 mg total alkaloid extract. The LCA model was developed with the application of SimaPro 8.1 software, for analysing the data resulted by the IMPACT 2002+ method. The IMPACT 2002+ impact assessment method, comprising of two sets of impact categories, eighteen midpoint-level impact categories (e.g., climate change, ozone and water depletion, eutrophication, and particulate matter formation), and three endpoint categories (damage to human health, ecosystem diversity, and resource availability). Based on the results of this study, it was found that green processes should be used for extraction of alkaloids, and based on the effects of chloroform used in the extraction process, the chemical materials with minimal damage to the environment must be replaced.