The most important method of haploid plant production is isolated microspores culture. To be successful in this method, genetic potential of cultivars is very important. In this research, ten Iranian hexaploid wheat cultivars (exclusive of MV17) on the basis of their characters such as heat, drought and salinity resistant have been selected and their responses to isolated microspore culture were evaluated. Meanwhile the effects of two pretreatments (cold and heat + chemical) and embryo induction media (NPB99 and CHB-2) on androgenic traits including embryogenesis, total plant regeneration and green plant regeneration were studied in selected cultivars. There were significant interactions between cultivars and pretreatments and also between cultivars and media in most cases. The interaction between cultivars Chamran and Navid with 21 days cold pretreatment produced the highest number of embryos and the interaction between cultivar Hamoon and 21 days cold and heat + chemical pretreatments produced the most green plant regeneration percentage. Also, the results showed that the interaction between cultivar Chamran and CHB-2 medium produced the highest number of embryos while the interaction between cultivar Hamoon and NPB99 medium had the highest green plant regeneration.