Seminal characteristics and blood biochemical attributes were studied in breeder roosters orally administered with sage extract (SG), an herbal extract well known to have potent antioxidant activities. Sixty roosters (34 weeks old) were randomly allotted to five treatment groups to receive no SG, or orally administered with 110, 210, 320, or 420 mg SG/kg liveweight for 8 weeks. Semen samples were evaluated weekly. Blood samples were taken fortnightly and a total of 21 biochemical indices were measured to unmask the effects of SG (especially the adverse ones) on the clinical profile. Excluding the sperm concentration and seminal content of thiobarbituric acid reactive substances (sperm membrane lipid peroxidation index), other seminal traits exhibited one of the linear, quadratic, or cubic responses to the various levels of SG. The most improvements in total live sperm number and sperm membrane integrity (as determined by the hypoosmotic swelling test) were observed in birds receiving 210 and 320 mg SG/kg liveweight, respectively. Serum testosterone level was generally higher (cubically, P = 0.015), but serum copper was lower (linearly, P = 0.014) in SG-administered birds. Birds receiving 320 and 420 mg SG showed a decreased content of C18 : 2(n-6) in sperm plasma membrane. Other biochemical attributes or sperm fatty acids were not affected. It seems that most improvement in the seminal characteristics could be achieved 5–6 weeks following the administration of 210 and 320 mg SG/kg liveweight without any apparent adverse effect on the blood biochemical indices. The improvements, however, could not be attributed to the antioxidative effect of SG. Although it is hypothesised that an increased serum testosterone might have been involved, the underlying mechanism(s) remain(s) to be clarified.
1. The aim of the present study was to compare wheat-based diets supplemented with arabinoxylanase, bile acid and virginiamycin with a maize-based diet in their effects on the performance of broilers. 2. The diets were fed to 5 groups of broilers. Group A received a maize-based diet (control), and groups B, C, D and E were fed on wheat-based diets. Diets C, D and E were supplemented with arabinoxylanase (150 mg/kg), bile acid (500 mg/kg) and virginiamycin (200 mg/kg), respectively. 3. A total of 450 1-d-old Ross 385 chicks were randomly divided into 5 groups, each with three pens as replicates (30 birds per pen). The experimental diets were given for 18 d. On d 18, three chickens from each group were slaughtered by cervical dislocation and the liver weight, and duodenal, jejunal and ileal lengths were measured for length and width of the villi and the height of crypts. 4. Arabinoxylanase, bile acid and virginiamycin supplementation significantly increased feed intake and daily gain but had no significant effect on feed conversion ratio. The liver weight was significantly greater in virginiamycin supplemented chicks as compared with those on the diet containing bile acid. 5. Virginiamycin inclusion increased full and empty caeca weight significantly compared with groups A and D. Group A had the lightest caeca. Significant effects of diets were observed on duodenal, ileal and intestinal length, but jejunal length was not affected by diet. The heights of duodenal and ileal, but not jejunal, villi were significantly affected by the treatments. The lengths of villi in groups B and E were significantly higher than other groups. The mean height of ileal villi in group A was significantly higher than in groups receiving the wheat-based diets. The mean width of duodenal and ileal villi was significantly affected by the ration. The height of crypts in group B was significantly higher than in group E. 6. It could be concluded that supplementation of the wheat-based broiler ration with virginiamycin had significant positive effects on gastrointestinal tract characteristics for absorption. Supplementation with bile acid reduced the weight of liver and caeca and the length of duodenum, jejunum, ileum and in small intestine and the height and width of villi.
In this study, 65,971 lactations on 41,842 cows in 64 herds were used to determine the association between dry period length (DPL) and lactation performance, lactation curve, calf birth weight, and the incidence of calving difficulty during the subsequent parity in Holstein dairy cows in Iran. The length of the dry period was classified into 7 categories: 0 to 35d, 36 to 50d, 51 to 60d, 61 to 70d, 71 to 85d, 86 to 110d, and 111 to 160d. Cows with the standard DPL (51 to 60d) produced more 305-d milk, fat, and protein over the next lactation compared with those with shorter dry periods. Shorter dry periods (0 to 35d and 36 to 50d) were associated with lower initial milk yield, steeper inclining and declining slopes of the lactation curve, and higher milk persistency compared with dry period length of 51 to 60d. Peak lactation was achieved later in cows with 0- to 35-d and 36- to 50-d dry period length than in those with dry period length of 51 to 60d. We also observed a relationship between DPL and calf birth weight: smaller calf birth weight was recorded with a dry period of 51 to 60d compared with longer dry periods. The incidence of calving difficulty did not differ in cows with 51- to 60-d dry period compared with cows with 0- to 35-d and 36- to 50-d dry periods. In conclusion, the results of this study did not support previous findings that suggested a shorter dry period could be beneficial to dairy production.
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