Lawsonia inermis is widely known as henna and belongs to Lythraceae family. It contains various phytochemica constituents in every part of plant and shows various pharmacological properties. Secondary metabolites are acts as antioxidants and these are very high in this plant species. In this research studies, ethanolic extract of Lawsonia inermis was performed in the blood sample culture for cytotoxicity and genotoxicity effects. The both of the effects of plant extracts were evaluated through measurement of mitotic index (MI), sister chromatid exchanges (SCE) and cell proliferation kinetics (CPK).  The blood sample culture was treated with control and various concentrations of extracts (500μg/ml and 1000μg/ml). The mean of MI, SCE and CPK for control were found to be 4.51,  5.19 and 0.52 respectively where as for 500μg/ml of Lawsonia inermis extracts are 4.33, 4.87 and 0.50 respectively and for 1000 μg/ml extracts are 4.18, 4.68 and 0.48 respectively. From the results, we can conclude that the MI and frequency of SCES of ethanolic plant extracts are remarkably similar to that of control which indicates that the Lawsonia inermis plant extracts have no substantial cytotoxicity and genotoxicity effects in cultured blood lymphocytes.