Background: So far, several interventions in the field of non-suicidal self-injury(NSSI) have been carried out under the title of schools silent crisis, however, no research has been found to develops and validates the integrated interventions approach on NSSI and its important consequences. Aims: The aim of the present study was to develop and validate an integrated interventional approach for NSSI, emotion regulation difficulties, mother-daughter relationship and negative automatic thoughts of adolescent girls. Methods: A quasi-experimental and pre-test,post-test research design with a control group were applied. The statistical population of the study was all first and second year high school students with NSSI. Using chain sampling, 32 participants were selected and randomly assiged to control and experimental groups in Ardabil in 2019-2020. Both groups were pre-tested and post-tested by Sansone, Wiederman, & Sansone Self-harm inventory (1998), Gratz & Roemer Difficulties in Emotion Regulation Scale ), Hollon & Kendall Automatic Thoughts Questionnaire (1980), and Fine, Moreland & Schwebel Parent-Child Relationship Survey-Mother (1985. Results: The results showed that the integrated interventional approach is effective in reducing the difficulties of emotion regulation, improving the mother-daughter relationship, reducing negative automatic thoughts, then reducing NSSI in adolescent girls )p<0.05(. Conclusion:The approach of integrated interventions is effective on research variables and could be used as an appropriate intervention by school counselors and therapists for adolescent girls with NSSI.