Purpose: Loss of the bracket-tooth bond is one of the most common complaints of patients during orthodontic treatment. Various factors play a role in preventing the loss of such a strong bond between the bracket and tooth, one of which is the maintenance of proper isolation and prevention of contamination of tooth surface with blood during the surgical exposure of the impacted tooth. In case of bleeding during disimpaction treatment, the use of hemostatic agents might decrease the odds of tooth surface contamination with blood, resulting in a strong bond. This study aimed to evaluate the effect of different hemostatic agents on the bond between the bracket and tooth. Materials and Methods: 144 standard stainless steel dies were divided into two groups. Each of them includes 72 dies. These groups were divided into 12 equal subgroups as well. In both groups, die spacer was applied to dies in 0, 3, 4, 5, 6 and 7 layers (each layer=5µ). In the first group, crowns were cemented with Zinc Phosphate and in the second group, Polycarboxylate was used for cementing. After that, the strength required for separating the castings from the dies was measured. Results: The difference among 12 subgroups analyzed by one-way ANOVA regarding Polycar- boxylate cement did not reach statistical significance. (P=0.95). A similar result was obtained with zinc phosphate cement (P=0.616). Likewise, the two-way ANOVA was carried out between the zinc phosphate and polycarboxylate groups. There was a significant difference between the average of two groups (P<0.001). Conclusion: Range of cement layer thickness that used in this study didn’t statistically significantly affect the force required to remove cemented cast copings. Although the castings cemented with Zinc Phosphate needed higher force to be dislodged from dies.