The aim of the study was to investigate the special effects of micronutrients (Fe, Zn, B, and Mn) under humic acid using condition on Thyme antioxidant activity. The trial conducted at the Experimental Fields and laboratory of the Agronomy Department, Faculty of Agriculture, Urmia University, West Azerbaijan, Iran, during 2015-2016. Experiment was set in randomized complete block design and in three replications. In the trial the treatments were included 3 g Fe L-1 , 3 g Mn L-1 , 4 g B L-1 and 2 g Zn L-1 in each time and humic acid (300 kg per hectare) were given to all plots. Spraying of the elements was done at three times: Some antioxidant properties of thyme leaves such as total phenolic content, total flavonoid content, DPPH radical scavenging activity, nitric oxide radical scavenging activity and Chain-breaking activity were determined. The results of first harvest showed that the highest flavonoid (0.84 mg quercetin/ g dry weight) and nitric oxide radical scavenging activity (58.28 %) related to B. The highest total phenol content (31.92 mg Gallic acid/g dry weight), DPPH radical scavenging activity (92.5%) and chain-breaking activity (74.25-Abs-3 /min/mg extract) related to Mn, Control and Zn, respectively. The results of second harvest showed that the highest total phenol content (40.05 mg Gallic acid/g dry weight), flavonoid (0.65 mg quercetin/ g dry weight) and DPPH radical scavenging activity (87.36%) related to B + Humic acid, and the highest chain-breaking activity (37.43-Abs-3 /min/mg extract) related to control. In conclusion B has positive effect on increasing the activity of antioxidant of the plant than other treatments.