No abstract

A simple, rapid and sensitive spectrophotometric method for determination of trace amounts of Promethazine hydrochloric in aqueous solution is described. The method is based on using the oxidative coupling reaction of promethazine hydrochloride with 4-amino 2-hydroxy benzoate reagent in acid medium in the presence of N-bromo Succinimide to produce an intense green colour, water soluble, product which exhibit maximum absorbance at 605.5 nm. Beer's law is obeyed over concentration range of (2-32µg/m1), with a molar absorptivity 7540.68 l/mol.cm, Sandel index of 0.04255 µg / .The average recovery is 100.7703 %, relative standard deviation of 0.3101 to 0.8474 % and D.L of 0.40825 µg/ml.

An easy, rapid and sensitive novel spectrophotometric method for estimation of trace amounts of Promethazine-HCl in aqueous solution is described. The method is based on using the oxidative coupling reaction of Promethazine-HCl with m-amino benzoic acid reagent at pH1.2 in the presence of N-bromosuccinimide to produce an intense green color, water soluble, product which exhibit maximum absorbance at 592 nm. Beer's law is obeyed over concentration range of (2-26 µg/ml), with a Ԑ 9.754 l/mol.cm, Coefficient determination (R 2 =0.9999 . a i . 9, the average recovery is 100.396 %, RSD does not exceed 0.3996 %, LOD of 0.1949 µg/m1 and LOQ of 0.5905 µg/m1. The proposed method was applied successfully for determination of PMZH in its pharmaceutical formulations.