Background and aims: Although vitamin D deficiency (VDD) is inversely associated with glucose intolerance, very limited data are available in Bangladesh. Thus, the study aimed to determine vitamin D status of Bangladeshi adults based on the glucose intolerance status. Materials & Methods: This cross-sectional study was carried out among 70 newly detected DM, age-group & sex-matched 76 newly detected pre-diabetes and 74 healthy control with normal glucose tolerance (NGT) based on American Diabetes Association, 2018 criteria. Participants were included consecutively from the Department of Endocrinology, Bangabandhu Sheikh Mujib Medical University (BSMMU) over a period of 1 year by convenient sampling to see serum 25(OH)D which was measured by high performance liquid chromatography (HPLC) method. Results: Vitamin D levels decreased with increasing degrees of glucose intolerance [NGT: 16.82±6.57; prediabetes: 15.54±8.58; T2DM: 11.01±6.03 ng/ml, p<0.001] but significantly lower in T2DM than other two groups [T2DM vs. prediabetes, p=0.001; T2DM vs. control, p<0.001; prediabetes vs. control, p=NS]. The highest frequency of vitamin D deficiency was among individuals with T2DM (64.3%) followed by NGT (29.7%) and prediabetes (28.9%) [p<0.001]. Only HbA 1 c had predictive association with vitamin D deficiency [OR (95%CI)=2.061 (1.178, 3.608), p=0.011]. Only DM but not pre-diabetes had predictive association with vitamin D deficiency compared with NGT [DM: OR (95%CI)=4.255 (2.117, 8.552), p<0.001; prediabetes: OR (95%CI)=0.963 (0.477, 1.946), p=0.916]. Conclusion: Vitamin D level was lower and frequency of vitamin D deficiency was higher in patients with DM. Only HbA 1 c and DM had predictive association with vitamin D deficiency.