Introduction: Today, due to the increasing application of silver nanoparticles in medical products, it is necessary to pay attention to the user's safety. There are three methods, namely, chemical, physical, and biological, used for the production of nanoparticles.Although the first two methods might introduce health hazards, the latter is hypothetically safe. In this study, we examined the histopathological changes in the cerebellum of neonatal Wistar rats induced by injection of toxic and nontoxic doses of silver nanoparticles, which were produced by green synthetic method and were compared with chemical silver nanoparticles.Methods: This study was a laboratory interventional study performed on 25 Wistar rats in the Animal Laboratory of Islamic Azad University of Shahrood. These rats were divided into five groups of the control group, the group with nonpoisonous injection of chemical nanoparticles, the group with nonpoisonous injection of biological nanoparticles, the group with injection of poisonous chemical nanoparticles, and the group with injection of poisonous biological nanoparticles. The rats were impregnated by the males of the same race and the cerebellum of their offspring was studied after birth. Results:We found that the injection of nonpoisonous chemical nanoparticles caused hyperemia, inappropriate size, and dark cytoplasm in some Purkinje cells. Also, injection of poisonous chemical nanoparticles caused hyperemia and cellular dispersion in the molecular layer, caused abnormal shapes, and reduced the number of cells in Purkinje cells. However, injection of poisonous and nonpoisonous biological nanoparticles did not alter cerebellum cells nor did it cause any inflammation or hyperemia. Conclusion:In contrast with chemical nanoparticles, biological nanoparticles have less significant effect on the cerebellum cells. K E Y W O R D Sbiological silver nanoparticles, cerebellum, chemical silver nanoparticles, histopathology, neonatal ratThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.