− An antimicrobial compound has been isolated from the leaves of Glochidion superbum. The compound was determined as methyl 3, 4, 5-trihydroxybenzoate (methyl gallate), based on ultraviolet (UV), infrared (IR), nuclear magnetic resonance (NMR) and mass spectroscopy (MS) analysis. The isolated compound exhibited potent antimicrobial activity against three clinical isolates of methicillin resistant Staphylococcus aureus (MRSA) by qualitative agar disc diffusion method and quantitative broth dilution method. Agar disc diffusion was done in a dose-dependent manner for each bacterial isolate at disc potencies of 25, 50, 100, and 150 µg/disc. The zones of inhibition were on average equal to 12.27, 14.20, 15.43, and 24.17 mm respectively. The inhibition zones were compared with that of vancomycin disc at 30 µg as a reference standard. The MIC and MBC values were 50 µg/mL and 100 µg/mL respectively. The results of anti MRSA activity were analyzed using one-way ANOVA with Turkey's HSD and Duncan test. In conclusion, methyl gallate which was isolated from G. superbum showed the inhibition activity against methicillin resistant S. aureus.
Introduction: The leaves of Glochidion superbum and other similar plants had been in use as a folk medicine by indigenous people in South East Asia for treatment of wound infections and diarrhoea. As recommended by WHO, studies on medicinal plants should include both identification of the chemical constituents and determination of their biological activities. Thus, the study aims to screen various local plant extracts for antibacterial activity and determine the extract fraction showing the highest antibacterial activity. Methods: In this study, Glochidion superbum leaves methanol extract was selected after preliminary antibacterial screening of this extract alongside extracts of Rennellia elliptica (leaves and roots) and Uncaria acida (stem and leaves) by the agar disc diffusion method against Escherichia coli ATCC 35218, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, and Bacillus subtilis ATCC 11778. Furthermore, methanol leave extract of Glochidion superbum was subjected to a qualitative phytochemical screening. The extract was fractionated using Vacuum Liquid Chromatography (VLC) method and the following fractions were obtained: methanol 2.2 g (4.7%), ethylacetate 38.3 g (81.4%) and ethylacetate:methanol 5.0 g (10.6%). Results: The ethylacetate:methanol 1:1 fraction showed the highest in vitro dose-dependent inhibitory activity at concentrations of 25, 50, 100 and 150 mg/mL against the growth of E. coli ATCC 35218, and S. aureus ATCC 25923. Conclusions: This research has demonstrated proof of concept that the plant extract of Glochidion superbum leaves has antibacterial activity against both gram-positive and gram-negative bacteria.
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