The aim of this study was to evaluate the effects of antibiotic residues in milk on growth, ruminal fermentation, and microbial community of dairy calves in their first 35 d of age. Twenty newborn Holstein bull calves were assigned to 1 of 2 treatments equally: milk replacer without antibiotics (control) and milk replacer plus 4 antibiotics: 0.024 mg/L of penicillin, 0.025 mg/L of streptomycin, 0.1 mg/L of tetracycline, and 0.33 mg/L of ceftiofur (ANT). Starter intake and fecal consistency scores of each calf were recorded on a daily basis. Body weight, withers height, body length, and heart girth were measured on d 1, 7, 14, 21, 28, and 35 before feeding in the morning. Rumen fluid was collected on d 15, 25, and 35 to determine ruminal pH, volatile fatty acids (VFA), and NH 3 -N concentrations. A total of 10 (5 per treatment) samples of rumen fluid taken on d 35 were analyzed for microbial community. Rumen tissues from the cranial ventral sac and cranial dorsal sac were collected from 8 calves of each group for morphology analysis on d 35 after being harvested. The results showed that calves in 2 treatments had similar starter intake, body weight, withers height, body length, heart girth, and average daily gain. The ANT group showed a lower diarrhea frequency in wk 4, and no differences were found for other weeks. Calves in the ANT group exhibited a greater concentration of acetic acid in the rumen and no differences for other VFA, total VFA, rumen pH, or NH 3 -N. As for rumen morphology, the length of papillae from cranial ventral sac of the ANT group was longer than that of the control group. The results of ruminal microbial community showed that antibiotic residues had minor effects on bacteria phyla and bacteria diversity. At the genus level, calves in the ANT group showed lower richness of Prevotella and higher richness of Acetitomaculum. In conclusion, antibiotic residues stimulated the development of ruminal papillae and increased the production of acetic acid in rumen, which might be caused by the influence of antibiotics on the ruminal microbial community.

The aim of this study was to investigate the effect of feeding milk replacer (MR) with two different antibiotics treatments on the gut microbiota of pre-weaning calves. Twelve (12) Holstein male calves at 1-day-old were randomly assigned to: milk replacer without antibiotics (CON), milk replacer plus low cocktail of antibiotics (LCA) concentration (penicillin 0.024 mg/L, streptomycin 0.025 mg/L, tetracycline 0.1 mg/L, ceftiofur 0.33 mg/L), and milk replacer plus a low concentration of single antibiotic (LSA; ceftiofur 0.33 mg/L). All the calves were harvested at 35-day-old, and the digesta from the ileum and colon was collected in addition to fecal samples. Samples were analyzed by 16S rRNA gene using Illumina MiSeq platform. Results showed that there were significant differences among treatments in the ileum, where LCA significantly reduced the relative abundance of Enterobacteriaceae (P = 0.02) especially Escherichia-coli (P = 0.02), while LSA significantly reduced the relative abundance of Comamonas (P = 0.02). In the colon and rectum, LSA treatment was significantly enriched with the class Bacilli, whereas the control group was significantly enriched with Alloprevotlla (P = 0.03). However, at the family level in the rectum LCA and LSA significantly reduced the relative abundance of Acidaminococcaceae (P = 0.01). Moreover, at the genera level in the colon, LSA significantly increased Prevotellaceae_Ga6A1_ group (P = 0.02), whereas in the rectum both of treatments reduced the relative abundance of Phascolarctobacterium (P = 0.01). In conclusion, the overall low cocktail of antibiotics concentration induced changes at different taxonomic levels; specifically the decrease in Escherichia-coli which might subsequently reduce the incidences of diarrhea in calves.

Feet and leg conformation traits are considered one of the most important economical traits in dairy cattle and have a great impact on the profitability of milk production. Therefore, identifying the single nucleotide polymorphisms (SNPs), genes and pathways analysis associated with these traits might contribute to the genomic selection and long-term plan selection for dairy cattle. We conducted genome-wide association studies (GWASs) using the fixed and random model circulating probability unification (FarmCPU) method to identify SNPs associated with bone quality, heel depth, rear leg side view and rear leg rear view of Chinese Holstein cows. Phenotypic measurements were collected from 1000 individuals of Chinese Holstein cattle and the GeneSeek Genomic Profiler Bovine 100 K SNP chip was utilized for individual genotyping. After quality control, 984 individual cows and 84,906 SNPs remained for GWAS work; as a result, we identified 20 significant SNPs after Bonferroni correction. Several candidate genes were identified within distances of 200 kb upstream or downstream to the significant SNPs, including ADIPOR2, INPP4A, DNMT3A, ALDH1A2, PCDH7, XKR4 and CADPS. Further bioinformatics analyses showed 34 gene ontology terms and two signaling pathways were significantly enriched (p ≤ 0.05). Many terms and pathways are related to biological quality, metabolism and development processes; these identified SNPs and genes could provide useful information about the genetic architecture of feet and leg traits, thus improving the longevity and productivity of Chinese Holstein dairy cattle.

Effects of milk feeding strategy and acidification on calf growth, metabolic traits, oxidative stress, and health were evaluated in the first 78 days of life. Holstein calves (N = 48; 12 calves/treatment) were assigned to 1 of the 4 treatments in a 2 × 2 factorial arrangement of milk feeding strategy [6 L/d (MOD) or 12 L/d (HIGH) of milk] and acidification [non-acidified milk (NAM) or acidified milk (ACM)] on day 2. Calves were bucket-fed milk as follows: 6 L/d from days 2 to 49 for MOD and 6 L/d from days 2 to 49, 12 L/d from days 7 to 42, and 8 L/d from days 43 to 49 for HIGH calves. All calves were then fed 4 L/d from days 50 to 56. Starter and water were available ad libitum, while hay was fed at 5% of starter from day 64. Calves were weighed, measured, and blood (except days 14, 42 and 56) sampled on days 2, 14, 28, 42, 49, 56, 63 and 78. Data were analyzed using Mixed PROC of SAS with time as repeated measurements. Fecal scores, checked daily, were examined by the logistic regression using a binomial distribution in GLIMMIX procedure. There were no three-way interactions observed for all the parameters. We detected a milk feeding strategy × time interaction for starter intake, body weight, ADG, ADG/ME, FE, structural measurements, and glucose. Although, overall (558.0 vs. 638.6 g/d), HIGH calves tended to consume less starter compared to MOD, significant differences were only observed in week 8. The HIGH calves had greater ADG during days 2–14 (1.12 vs. 0.75 kg/d) and tended to have greater ADG on days 15–28 (0.79 vs. 0.55) and 29–42 (0.86 vs. 0.60) and lower on days 57–63 (0.11 vs. 0.38) compared to MOD calves. The HIGH calves had greater BW from days 28 to 78 compared to MOD, while NAM were bigger compared to ACM calves from days 49 to 78. The HIGH calves had lower overall feed and metabolizable energy efficiencies compared to MOD. Except for BW and heart girth, no milk acidification × time interaction was observed for starter intake, ADG, FE, or ADG/ME. Blood glucose in calves fed NAM-HIGH and ACM-HIGH were greater compared to those fed moderate milk volumes on day 28 only. Albeit, feeding strategy had no effect, calves fed ACM had lower likelihood of experiencing diarrhea (odds ratio = 1.32; 95% confidence interval: 1.018–1.698) compared to those fed NAM. Overall milk feeding strategy had no effect on growth, while milk acidification reduced growth in calves, despite lowering the likelihood of experiencing diarrhea.