The purpose of the experiment is an investigate the association of walnut oil with lipid profiles, glucose as well as total proteins and assessment its side effect on some sensitive organs such as the liver and spleen tissues. The experiment divided into two main groups are treated group with walnut oil and the control group, where the former divided into two subgroups 0.25 and 0.5 ml of oil per each. We found a statistically non-significant difference between treated and control groups (for total protein, lipid profile, and glucose). There was no change in total protein, but cholesterol decreased by 0.25 ml but a little increased by 0.5 ml. HDL was increased for the treated group. While on 0.25 ml LDL decreased in treated animals, on another hand no change for 0.5 ml. Also, no change for VLDL between treated and control. The only triglyceride was increased but non-significant for the treated group compared with the control. Both doses decreased in treated animals for glucose. We also found an increase in whole-body weight and on sensitive organs such as the liver and spleen. Even no change in a histological study for the mentioned organs. The conclusion: By walnut oil, all parameters have changed despite the treated group was normal without any induced diseases. So, recommended the researchers induce the disorders in the liver and assessment the extracted oil on the lipid profile.