The current study examined the genotoxic effect of the biosynthetic zinc oxide nanoparticles ZnONPs using the Vitex agnus-castus plant, which contains effective compounds that inhibit the growth of pure cancer cells. The phenotypic and structural characteristics of the biosynthesized nanoparticles were detected using scanning electron microscopy (SEM). Most of the ZnONPs were dense and spherical with sizes ranging from (20-61) nanometers, on the cervical cancer cell line Hela and compared with the normal line Human Foreskin Fibroblast cells (HFF) using green dye. Comet assay technique and genotoxicity experiments were carried out at the Iraqi Center for Cancer Research / Al-Mustansiriya University. The results of this study showed the presence of damage to the DNA of cervical cancer cells Hela, which stopped cell division and entry. Cells are in the stage of programmed death a result of the release of reactive oxygen radicals (ROS) and changing cell functions by linking the nanomaterial to the genetic material. This gives hope for discovering a successful treatment to eliminate cervical cancer Hela or any type of cancer using methods Biosynthesis of nanoparticles.

The cytotoxic impact of biosynthetic zinc oxide nanoparticles (ZnONPs) was investigated using Vitex agnus-castus, which has been shown to have Effective compounds that suppress cancer cell proliferation. Zinc oxide nanoparticles were biosynthesized in the laboratories of the Biology department /College of Education for the Pure Sciences /University of Diyala. The phenotypic and structural characteristics of biosynthetic nanoparticles were identified using scanning electron microscopy (SEM). The majority of ZnONPs are dense and spherical in shape, with diameters ranging from (20-61) nm, and were discovered on the cervical cancer cell line Hela and compared to the normal line Human Foreskin Fibroblast cells (HFF) using the MTT stain test (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium ). Cytotoxicity experiments were conducted at the Iraqi Center for Cancer Research / Al-Mustansiriya University. This study showed inhibitory activity on Hela cervical cancer cells, where the highest inhibition rate reached 93.6% at a concentration of 200 µg/ml. This raises the prospect of finding a viable therapy for cervical cancer (Hela) or any other malignancy using nanoparticle manufacturing technologies. Keywords: Biosynthetic, Green nanoparticles, Cancer cell, Hela, GC-MS.