Medical science has paid a great deal of attention to green synthesis silver nanoparticles (AgNPs) because of their remarkable results with multidrug-resistant bacteria. This study was conducted on the preparation of AgNPs, using the teucrium apollinis extract as a reducing agent and a capping ligand. The AgNP produced was stable in room condition up to 10 weeks. The AgNP was characterized using UV-visible absorption spectroscopy (UV-Vis), attenuated Fourier transform infrared (ATR-FTIR), transmission electron microscopy (TEM), and dynamic light scattering (DLS). The study confirms the ability of teucrium apollinis to produce AgNPs with high stability. The influence of pH was studied over a pH range of (2–12) on the stability of synthesized AgNPs. The best value of pH was 7.2, where AgNP showed a good stability with high antibacterial activity against Pseudomonas aeruginosa. AgNP synthesis is confirmed by a strong peak in the UV-Vis due to surface plasmon resonance (SPR) at 379 nm. Based on TEM findings, monodispersed AgNP has a spherical shape with a small size of 16 ± 1.8 nm. In this study, teucrium apollinis extract was used for the first time, which could be a good environmental method for synthesizing AgNP, which offers a possible alternative to chemical AgNPs.

Silver nanoparticles (AgNPs) have been boosted in the medical field because of using suitable stabilized ligands leading to create specific shapes and sizes. AgNPs in this study are prepared in one-step using vitamin C (VC) and kojic acid (KA) as stabilized ligands in presence of sodium borohydride (NaBH4). As known, the Food and Drug Administration (FDA, USA) approved the use of KA for dermatological treatment purposes. Also, vitamin C as an antioxidant has reduced cancer diseases. The AgNPs are sufficiently stabilized by these biomolecules to remain dispersed in an aqueous solution for up to 12 weeks under room conditions. The AgNPs were characterized using UV-Visible absorption spectroscopy (UV-Vis), transmission electron microscopy (TEM), dynamic light scattering (DLS), and attenuated Fourier transform infrared (ATR-FTIR). The produced AgNPs were spherical and monodispersed with a size diameter range of 11–15 nm based on the TEM results. The antibacterial activity of AgNPs was tested against Escherichia coli (E. coli) and Streptococcus aureus (S. aureus) at different concentrations (4 µg/ml, 8 µg/ml, 15 µg/ml, and 30 µg/ml). The finding results demonstrated that both these AgNPs significantly reduced bacteria growth, especially at the highest concentration of 30 µg/m. The VC-AgNP was highly active on the E. coli and S. aureus even at a lower concentration of 4 µg/ml. VC/or KA-AgNPs provided more encouraging results than when VC and KA were used alone, which would eventually allow VC dosages to be reduced in the future, which could be more effective against many diseases in the future.