The newly developed molecular biology approach expanding the genetic code was used to incorporate the non-canonical amino acid dihydroxyphenylalanine for ne-tuning of proteins. Further, the congener protein was enzymatically converted to form quinone for strain promoted click chemistry. The reaction yields a single product with de ned stereochemistry and temporally controlled conjugation with bicyclonon[6.1.0]-4-yne (BCN) as well as dibenzocyclooctyne-PEG-Fluor 545. The promising bioconjugation of congener protein with dibenzocyclooctyne-PEG-Fluor 545 was used as a uorescent marker for selective cell imaging and detection of programmed cell death in EAhy926 cells.

No abstract

The newly developed molecular biology approach expanding the genetic code was used to incorporate the non-canonical amino acid dihydroxyphenylalanine for fine-tuning of proteins. Further, the congener protein was enzymatically converted to form quinone for strain promoted click chemistry. The reaction yields a single product with defined stereochemistry and temporally controlled conjugation with bicyclonon[6.1.0]-4-yne (BCN) as well as dibenzocyclooctyne-PEG-Fluor 545. The promising bioconjugation of congener protein with dibenzocyclooctyne-PEG-Fluor 545 was used as a fluorescent marker for selective cell imaging and detection of programmed cell death in EAhy926 cells.