Our study is designated to determine the impact of SB in the induction HDPs, including AvBD-10 and CATH-B1, accompanied by two different inactivated H9N2 vaccines and their effect on body performance. One hundred fifty, day-old chicks were separated into five groups (30 chicks for each, three replicates): groups A and C were vaccinated with classical avian influenza H9N2 and developed H9N2P inactivated vaccines, respectively, but groups B and D were treated with sodium butyrate (SB) by a dosage of 1gm/liter of drinking water daily till the end of the trail, and these groups (B and D) received the same type of vaccines as they given to group A and C respectively, while group E is a control group. The results illustrated that SB improved the AvBD-10 level significantly in the treated group (B and D) at 14 days in comparison with groups A and E, but without significant with group C. Whereas at 35 days, this improvement occurred distinctly in treated groups B and D. The same improvement revealed with CATH-B1 at 35 days of experiments. Moreover, the supplementation of SB improved FCR in groups B and D at 35 days of the experiment, respectively, but no influence on WG between all groups at the same age. Thus, we concluded that supplemented SB enhanced innate immunity by stimulating the induction of AvBD-10 and CATH-B1. Also, these supplementations improved FCR but did not influence WG.