This research has been carried out on the effect of some biochemical parameters on women with polycystic ovaries. The presented work aims to determine the levels of a few biochemical parameters in females with polycystic ovaries syndrome (PCOS) attending the Department of Biotechnology, College of Science, University of Baghdad’s. The study comprised 35 women between 18 and 35 who had PCOS based upon complete Rotterdam criteria. The control group comprised 35 healthy females of one age who had a regular menstrual cycle. The results indicated that females with PCOS have been obese or overweight when their body mass index (BMI) was more than 30. Insulin, fasting blood sugar (FBS), glycated hemoglobin (HbA1c%), and homeostatic model assessment for insulin resistance (HOMA-IR) have all been higher (≤ 0.05) in females with PCOS in comparison with a control group, showing that insulin resistance (IR) has been present. Total cholesterol (TC) and low-density lipoprotein (LDL) levels have been higher (≤ 0.05). Still, very-low-density lipoprotein (VLDL) and triglycerides (TG) levels were within the normal reference range without considerable differences compared to the control group. High-density lipoprotein (HDL) has been lower than the control group. According to this work, the majority of females with PCOS had a high BMI. Those patients showed signs of IR. It was discovered that the patient had dyslipidemia. Those results vary from previous studies that found no evidence of IR. The primary goal of this work is to assess the effects of homocysteine and vitamin D in females who have PCOS, and the findings revealed a significant increase in the homocysteine levels and a considerable drop or deficiency in vitamin D in Iraqi females who have PCOS.
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