The depletion of fossil fuel resources and environmental pollution are two major crises that are currently faced by mankind. As the solution for the crises, an additive liquid like Tripmexx is introduced into automotive industries to increase performance of internal combustion engine, hence reducing bad emission to the environment. The objective of the study is to investigate the effect of various Tripmexx mixed into 80% diesel and 20% biodiesel (B20) with an amount of 0.1 ml (B20-0.1), 0.2ml (B20-0.2), and 0.3ml (B20-0.3) compared to conventional pure diesel. The experiment was carried out by using single cylinder, four-stroke diesel engine, and conducted with constant speed at range of 1000, 1500, and 2000 rpm with various load range from 2Nm to 6Nm. For constant speed 2000 rpm, the B20-0.3 is in lower brake specific fuel consumption value compared to the diesel fuel by 40% on average. The blend B20-0.3 produce higher brake thermal efficiency reading compared to diesel fuel by 5%. Besides, the carbon monoxide and hydrocarbon product of B20-0.3 is lower than diesel fuel by 92% and 0.002% respectively. However, the nitrogen oxide and carbon dioxide produced by B20-0.3 is higher than diesel fuel by 68% and 50% respectively. In overall, the B20-0.3 shows the best results for all measured parameters at all engine test due to consideration of green fuel.