Myostatin mutations in mice and cattle are associated with increased muscularity, suggesting that myostatin is a negative regulator of skeletal muscle mass. To test the hypothesis that myostatin inhibits muscle cell growth, we examined the effects of recombinant myostatin in mouse skeletal muscle C2C12 cells. After verification of the expression of cDNA constructs in a cell-free system and in transfected Chinese hamster ovary cells, the human recombinant protein was expressed as the full-length (375-amino acid) myostatin in Drosophila cells (Mst375D), or the 110-amino acid carboxy-terminal protein in Escherichia coli(Mst110EC). These proteins were identified by immunoblotting and were purified. Both Mst375D and Mst110EC dose dependently inhibited cell proliferation (cell count and Formazan assay), DNA synthesis ([3H]thymidine incorporation), and protein synthesis ([1-14C]leucine incorporation) in C2C12 cells. The inhibitory effects of both proteins were greater in myotubes than in myoblasts. Neither protein had any significant effects on protein degradation or apoptosis. In conclusion, recombinant myostatin proteins inhibit cell proliferation, DNA synthesis, and protein synthesis in C2C12muscle cells, suggesting that myostatin may control muscle mass by inhibiting muscle growth or regeneration.

On 7 Feb 2021, a catastrophic mass flow descended the Ronti Gad, Rishiganga, and Dhauliganga valleys in Chamoli, Uttarakhand, India, causing widespread devastation and severely damaging two hydropower projects. Over 200 people were killed or are missing. Our analysis of satellite imagery, seismic records, numerical model results, and eyewitness videos reveals that ~27x106 m3 of rock and glacier ice collapsed from the steep north face of Ronti Peak. The rock and ice avalanche rapidly transformed into an extraordinarily large and mobile debris flow that transported boulders >20 m in diameter, and scoured the valley walls up to 220 m above the valley floor. The intersection of the hazard cascade with downvalley infrastructure resulted in a disaster, which highlights key questions about adequate monitoring and sustainable development in the Himalaya as well as other remote, high-mountain environments.

ABSTRACT. We present a comprehensive review of the status and changes in glacier length (since the 1850s), area and mass (since the 1960s) along the Himalayan-Karakoram (HK) region and their climatechange context. A quantitative reliability classification of the field-based mass-balance series is developed. Glaciological mass balances agree better with remotely sensed balances when we make an objective, systematic exclusion of likely flawed mass-balance series. The Himalayan mean glaciological mass budget was similar to the global average until 2000, and likely less negative after 2000. Mass wastage in the Himalaya resulted in increasing debris cover, the growth of glacial lakes and possibly decreasing ice velocities. Geodetic measurements indicate nearly balanced mass budgets for Karakoram glaciers since the 1970s, consistent with the unchanged extent of supraglacial debris-cover. Himalayan glaciers seem to be sensitive to precipitation partly through the albedo feedback on the short-wave radiation balance. Melt contributions from HK glaciers should increase until 2050 and then decrease, though a wide range of present-day area and volume estimates propagates large uncertainties in the future runoff. This review reflects an increasing understanding of HK glaciers and highlights the remaining challenges.

ABSTRACT. Surface digital elevation models (DEMs) and slope-related estimates of glacier thickness enable modelling of glacier-bed topographies over large ice-covered areas. Due to the erosive power of glaciers, such bed topographies can contain numerous overdeepenings, which when exposed following glacier retreat may fill with water and form new lakes. In this study, the bed overdeepenings for �28 000 glaciers (40 775