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Under the present study, an attempt was made to characterize rhizobacteria i.e. Pseudomonas and Bacillus species isolated from rhizosphere of carnation to evaluate their growth promoting effect on carnation so as to select and develop more effi cient indigenous plant growth promoting and disease suppressing bioagents of specifi c soil type and specifi c plant type. Maximum strains of Pseudomonas and Bacillus sp. showed signifi cant antimicrobial activities against most of the microorganisms tested. On the basis of in vitro antagonistic activities ,the best strains were selected and used in fi eld trial to study the infl uence of these strains on the growth of carnation. Results have shown marked effect on growth parameters and disease incidence has also been reduced signifi cantly.

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Microorganisms such as Escherichia coli, Pseudomonas aeruginosa, and Bacillus subtilis isolated from cervices of infertile females possessed spermicidal activity. They also agglutinated the human spermatozoa in vitro and showed tail-to-tail agglutination. Cell-free supernatant of these organisms was found to be spermicidal but did not agglutinate spermatozoa in vitro. Spermicidal activity was increased with increase in age of the culture.