A B S T R A C TA total of 194 milk samples from clinically mastitis cattle cows were collected from Giza, Monofia, Fayoum, Ismailia, and Beni-Suef Governorates. All samples were collected during the period from December 2016 till June 2017. Bacteriological study gave a total of 29 positive strains of Escherichia coli (E.coli) in the rate of (14.9%) from all collected samples. Twelve E.coli isolates were identified from cultured samples in a single manner (6.2%) and was isolated with Staphylococcus aureus (S.aureus) by 8/194(4.1%), with Streptococcus species (Strept. spp) by 3/194(1.57%), meanwhile it was isolated with S.aureus and Strept. spp. by 6/194(3.1%). On the other hand 18 clinical mastitic milk samples were showed no growth of any pathogenic microorganisms on ordinary and specific used media of bacteriology from all investigated milk samples by (9.3%). It was observed that several serotypes were recovered from clinical cases of milk sample with different E.coli infection as O27, O146, O125, O126, O111, O20, and O157. Concerning the sensitivity test to choice the suitable antibacterial drug(s) for treatment clinical mastitis in cattle cows the data revealed that ,Cefiquinom, Gentamycin and Amoxicillin +Clavulinic acid were the antibacterial drugs of first choice that could be used to overcome a great number of single isolated E.coli causing clinical mastitis. Vice versa, the resistant antibiotics for single E.coli infection causing clinical mastitis were Amoxicillin, Ampicillin, and Neomycin. Studied strains were gave a positive results for virulence E.coli genes: phoA ,ompA and fimH in 5 examined strains (100 %),classified as follow :(two strains of O27/28.6%) ,( one strain of O125/14.3%) ,( one strain of O126/14.3%) , and (one strain of O146/14.3% ). while Stx1 and Stx2 virulence genes were detected in only 2 studied strains of E.coli in a total percentage of 28.6 %, divided into, O111(14.3%) and O157(14.3%).

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A B S T R A C TA total of 213 milk samples from clinically mastitic cattle cows were collected from different Governorates of Egypt and transferred in ice box as soon as possible to Bacteriological lab. in Animal Reproduction Research Institute (ARRI) in Giza Governorate (AL-Haram) for bacteriological examination of most important pathogens causing clinical mastitis with special references for isolation and strict identification of Salmonella species. All samples were collected during the period from December 2016 till July 2017 from governorates of Egypt. The bacteriological investigations revealed that 8 (3.7%) of Salmonella isolates were identified biochemically from all examined samples. Serological study showed that a total of 5 (2.3%) of Salmonella isolates were typed as Salmonella Typhimurium. Two strains of Salmonella Typhimurium were isolated singly in the rate of (0.93%) from all examined samples, also another two strains of Salmonella Typhimurium were isolated mixed with Staph aureus in the rate of (0.93%), meanwhile only one strain of same species was isolated mixed with E.coli in the rate of (0.47%). Cefiquinom and Enrofloxacin were sensitively in the rate of (100%), Ampicillin, Chloraphincol, Cloxacillin were resistance in the rate of (100 %) to Streptomycin and Amoxicillin. The molecular examination confirmed that all 5 examined serotyped strains were Salmonella Typhimurium. Virulence genes invA, hilA, avrA, were detected in examined sample by 100%, meanwhile sopE, ssaQ, and fimH genes were not detected by zero%. The objectives of the present study was to investigate the occurrence of Salmonella serotypes as well as to determine the frequency distribution of Six virulence genes (invA, hilA, avrA, ssaQ, sopE and fimH) in salmonella isolates from cattle clinical mastitic milk, in addition to determine the drugs of choice for treatment of most Salmonella strains causing cattle clinical mastitis.

A number of 271 apparently healthy camels of both sexes at different ages were used in this study during a period from April 2016 to May 2017. Samples (271 serum samples, 30 milk samples , 21 tissue specimen and lymph nodes) were obtained from slaughtered camels in different localities (Cairo, Giza, El Sharkyia, El Behira, Matroh). Three serological tests were applied including Buffered Acidified Plate Test (BAPT), modified Rose Bengal Plate Test (mRBPT), and Tube Agglutination Test (TAT). The prevalence of reactors for brucellosis was 9.5%, 8.8% and 7.7% respectively. All samples confirmed by using Rivanol Test (Riv.T) and Immunochromatographic assay (ICA) with 8.5% and 9.2% prevalence respectively. All collected milk samples were Negative by MRT (0/30). ICA or (LFA) is highly sensitive, accurate and specific diagnostic assay since it directly detects antibodies of Brucella organism and is considered as rapid confirmatory test. Culturing from 21 tissue samples and L.N revealed positive isolates as 5/21 (23.8%). The isolated strain was identified biochemically and by Polymerase Chain Reaction (PCR) with 498 bp and revealed predominance of Brucella abortus in all isolates as 5 (100%). This work aims at providing an overview on diagnostic investigations, as brucellosis has an economic impact on the production and reproduction in camels.

This study aimed to assess the relation between the morphometric and histological attributes of the epididymis, and epididymal semen features in mature dromedary camels. The testes with the attached epididymis (n=50) were collected from adult camels along the rutting season from December to April. The separated epididymis was evaluated for weight and length prior to the dissection. Samples from each epididymal segment were processed for histomorphometric examination. Epithelial height, luminal diameter, tubular diameter, stereocilia height, muscular coat thickness and histogram of the intra luminal content were measured. The harvested epididymal semen was evaluated for motility, concentration and livability. The intra-luminal cellular contents (histogram in pixels), epithelial and stereocilia heights, and muscular coat thickness were maximal in the epididymal head. The epididymal tail showed wider luminal and tubular diameters than head and body. The tubular diameter and histogram were positively correlated (p<0.05) with sperm motility in the head and body segments. The length of head epididymal segment was negatively correlated with sperm concentration. Epithelial height, stereocilia height were negatively correlated with sperm motility in epididymal body. In conclusion, the histomorphometry aspects of the epididymis eminently impacts spermatozoa features in dromedary camel.