Abstract:Background -The use of physical means as an aid for modern medicine in the champion against pathogenic microorganisms holds new approach that recently have begun to be widely recognized. The use of an additional physical means, alternating currents, introduced to inhibit bacterial growth and enhance disinfectant potency. The present study aimed to determine the best frequency of alternating currents in prevention of bacterial growth and to detect the efficacy of alternating currents on disinfectant bactericidal potency. Material and Methods -Electric field strength of 6 and 10 V/cm 2 at 50 KHz, 1 MHz, 10 MHz and 20 MHz was applied continuously during lag phases of staphylococcus aureus and pseudomonas aeroginosa. Then Changes in bacterial growth were investigated by the time kill method. Efficacy alternating currents on the current disinfectants bactericidal potency (microzed, deconex, Dettol and glutaraldehyde) were evaluated by MIC and MBC. Results -Alternating current at the low voltage and high frequency (10 V/cm 2 at 20 MHz) reduced the growth of S. aureus and P. aeruginosa effectively. Electric field strength of 10 V/cm 2 at 20 MHz showed a better effect on the low level disinfectants such as Dettol and Deconex compared to the high level disinfectants such as Glutaraldehyde, Microzed GP-H and Aniosyme. Conclusion -Detection of a suitable form of alternating current is necessary in the future. This method may be applied as a complementary for eliminating of conductive and semi-conductive surfaces of hospital and increase disinfectant bactericidal potency.

BACKGROUND Basal cell carcinomas (BCCs) are locally invasive periocular skin cancers affecting lower eyelids more than upper eyelids. The purpose of this study was to describe techniques used for lower eyelid reconstruction after extended excision of BCC. METHODS Eight referred patients with BCC who underwent lower eyelid reconstruction were enrolled. The tumor was surgically excised with sufficient margins by one surgeon. Defects were repaired by subdermal tunnel between lateral border of defect and insertion site of lateral cantus. RESULTS Eight patients aged 45 to 75 years were followed up for 6 months. After follow up, adequate viability of the grafts, satisfactory functional and good cosmetic results was noticed in all patients. One patient complained of irritation at the site of surgery. No total or partial necrosis, hematoma, or infection were observed in flaps, and no additional surgery was needed. CONCLUSION The present novel surgical procedure was useful to close full thickness defects in the lower lid to preserve the function of the lower eyelid and a good aesthetic outcome.

BACKGROUND Microvascular surgery is one of the most important parts of reconstructive surgery. In the present study, the effect of ethanol on microvascular diameter and prevention of thrombosis was evaluated. METHODS Totally, 80 vessels including 40 arteries and 40 veins in right and left ears of 20 adult rabbits were enrolled. Seven days after injection of ethanol to rabbit ear vessel, vessel diameter and thrombosis rate post-iced saline challenge were documented and compared to normal saline injection in contralateral ear as a control group. RESULTS Vessel diameter in both arteries and veins in ethanol group was significantly larger than normal saline control group, and patency rates due to preventive effect of ethanol were also significantly higher in the ethanol group after iced saline challenge. CONCLUSION Pretreatment with ethanol can enlarge vessel diameter and play a preventive role on thrombosis after iced saline challenge.

Nasiri et al.: P371: Utilization of alternating currents as a novel procedure for increasing of disinfection efficacy against Staphylococcus aureus and Pseudomonas aeroginosa. Antimicrobial Resistance and Infection Control 2013 2(Suppl 1):P371.