Undoubtedly, the snake anti-venom is a medication made up of antibodies used to treat its bites by venomous snakes. Repeated administration of snake venoms from Spitting cobra, in gradual sub-lethal doses into horses has been reported to cause various ill health conditions to recipient animals beside loss in the antibody titer in the plasma harvested from immunized horses. It could accordingly, propose some amendment in the immunization schedule using different adjuvants to choose the best one giving the maximal amount of antibody beside its max safety measures. Montanide ISA is an alternative to CFA for hyper-activation of immune response in rabbits beside it is considered as adjuvant with high safety margin.