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Egypt has one of the highest prevalence rates of hepatitis C virus (HCV) in the world. Occupational exposure to HBV and HCV is a well-recognized risk for health care workers (HCWs). The current study conducted to determine the prevalence of HBV and HCV among HCWs in governmental hemodialysis units in Daqahlyagovernance (DG). Cross-sectional design was utilized to carry out this study. This study was conducted at 33 governmental hemodialysis units. The total number of the study participants were 1043 HCWs. Structured interviews were run to assess socio-demographic, and occupational characteristic of HCWs; their health history, and laboratory investigations were carried outto estimate the prevalence of HBV and HCV among themwhich were firstly for HCV (hepatitis C virus antibody 3rd generation);secondly for HBV (HBsAg, and hepatitis B surface antigen).Results indicated that46.8% of HCWs aged 30 to less than 40 years, 45.6% of HCWs were university education, 94.7% of HCWs received HBV vaccination, finally prevalence of HCV was 5.4%, while prevalence of HBV was 0.48%. the researcher could be concluded that: prevalence of both HBV and HCV among heath care workers at governmental hemodialysis units in Dakahlya governorate is very low; with the highest one for HCV than HBV. Finally, this study recommended that: Regular screening for HBV and HCV infection status among HCWs specially in hemodialysis units should be performed.