Background: The need to identify and characterize new antimicrobial agents is important due to the increasing development of resistance by microorganisms to the existing antimicrobial agents. Aim: This study examined the efficacies of Mangifera indica on Escherichia coli and Staphylococcus aureus. Method: Three parts (leaf [L], root [R], and bark [B]) of the plant were analyzed. The extraction of the samples was performed by aseptically grinding the samples, dissolving in absolute ethanol, and filtering through whatman filter paper. The efficacy of the extracts bothsingle and combined was determined using agar well diffusion assay with gentamycin [10 µl] (E. coli) and vancomycin [30 µl] (S. aureus) as control antibiotics. Results: The higher concentration (C 2 = 3.0 g/ml) showed more antibacterial effectiveness than the lower concentration (C 1 = 1.5 g/ml) against both bacterial isolates with significant differences (P < 0.05) in all extracts except for single extracts (E. coli dry leaf extract; fresh bark extract), double extracts (S. aureus: dry and fresh leaf extracts) and triple extract (E. coli and S. aureus dry extracts). For the single extracts the bacteria has the following significant results: E. coli L