The salticid spider Habrocestum graecum Dalmas, 1920, until now only known from Greece, is for the first time recorded in Italy. Observations on ecology and behavior are also reported and pictures of its habitus and genitalia are provided. Furthermore, the first DNA barcode sequence for H. graecum is produced and made publicly available. The species has been observed in Puglia, in South-Eastern Italy, and a trans-Ionian dispersal pattern is most likely the cause of its presence both in Greece and Southern Italy, as reported for other taxa with similar distribution in different animal groups.