This article reports the results of a study that aimed to analyze the level of accessibility of the websites of Colombian universities under the accessibility guidelines for web content, WCAG 2.1. The web portals of 11 universities categorized in the Q1 and Q2 quartiles of the U-sapiens 2020-1 Ranking were analyzed. The results show that no university met all the success criteria, and the most accessible only reached 71.79 % of them. No relationship was found between the accessibility of web pages and the ranking of universities in the U-Sapiens 2020-1 ranking. This indicates that the websites of the universities studied are not fully accessible. Although there are regulations that promote web inclusion for all people, regardless of their disability status, it is necessary to promote educational campaigns that teach and motivate designers, digital content creators, and programmers to consider when developing websites.