BackgroundRecent studies have found associations between the gene encoding pregnancy associated plasma protein-A2 (PAPP-A2), a protease of insulin-like growth factor binding protein -5 (IGFBP-5), and measures of female reproductive performance in cattle. The purpose of the present study was to test the effects of Pappa2 deletion on reproduction in mice.FindingsWe measured the fertility and offspring growth of Pappa2 deletion females, and also performed reciprocal matings (i.e., deletion males mated to control females) to control for the effects of offspring genotype. Ovarian and testicular IGFBP-5 levels were measured by Western blotting. As expected, deletion of Pappa2 increased ovarian IGFBP-5 levels. However, Pappa2 deletion in females had no effect on the interval between pairing and the birth of the first litter, the interval between the births of the first and second litters, or litter size. Offspring weight was lower in the offspring of Pappa2 deletion females, but effects of similar magnitude were observed in the offspring of Pappa2 deletion males, suggesting that the effects were due to heterozygosity for the deletion in the offspring. Pappa2 deletion in males had no effect on litter size or the interval between pairing and the birth of the first litter. However, the interval between the births of the first and second litters was significantly longer in deletion males.ConclusionsPappa2 deletion had no effect on female reproductive performance. In contrast, Pappa2 deletion had subtle effects on male fertility, although the underlying mechanism remains to be elucidated.

All over the world there are more vegetarians than in previous years due to many reasons. One of them is positive influence on health what is shown in the latest research. The aim of the article is to review the influence of vegetarian diet on frequent diseases such as: hypertension, obesity, osteoporosis. An analysis of scientific papers from Pubmed and Google Scholar was performed, which most accurately described the issue of impact vegetarian diet on human health. The following keywords have been used in the search: Vegetarian, hypertension, obesity, osteoporosis: vegetarian, hypertension, obesity, osteoporosis. The researchers shown than vegetarians have lover systolic blood pressure and diastolic blood pressure by 10 and 5 mm Hg, they also lower overall mortality by 10-15%, risk of heart failure and by~40%, stroke by~35% and coronary events by 20%. Vegetarians also have statistically lower body mass index. What's more in this diet is more energy efficient thanks too low food energy density and helps keeping leptin on adequate level. Research has shown than vegetarians have lower mineral bone density than non-vegetarian, although some studies has shown that risk of fractures in vegetarians and non-vegetarians was not affected by diet alternation. Based on the results of the studies above vegetarian diet contributes to lower blood pressure and preventing from cardiovascular diseases. It helps to avoid obesity and to get rid of weights also doesn't have negative impact on osteoporosis.

Background Lactation results in substantial maternal bone loss that is recovered following weaning. However, the mechanisms underlying this recovery, and in particular the role of insulin-like growth factor 1 (IGF-I), is not clear. Furthermore, there is little data regarding whether recovery is affected by advanced maternal age. Methods Using micro-computed tomography, we studied bone recovery following lactation in mice at 2, 5 and 7 months of age. We also investigated the effects of reduced IGF-I availability using mice lacking PAPP-A2, a protease of insulin-like growth factor binding protein 5 (IGFBP-5). Results In 2 month old mice, lactation affected femoral trabecular and cortical bone, but only cortical bone showed recovery 3 weeks after weaning. This recovery was not affected by deletion of the Pappa2 gene. The amount of trabecular bone was reduced in 5 and 7 month old mice, and was not further reduced by lactation. However, the recovery of cortical bone was impaired at 5 and 7 months compared with at 2 months. Conclusions Recovery of the maternal skeleton after lactation is impaired in moderately-aged mice compared with younger mice. Our results may be relevant to the long-term effects of breastfeeding on the maternal skeleton in humans, particularly given the increasing median maternal age at childbearing.

Introduction: Alcohol is one of the most widely-used stimulants. It’s easily accessible, most socially accepted and usually not associated with potential harm it can do. Except for its addictive effect, it has a strong influence on humane systems.  In this analysis the influence of alcohol on the cardiovascular system will be examined. As it is not obvious whether alcohol usage should be only associated with harmful impact on cardiac activity, different types of alcoholic beverages should be examined.  In this work three different sub-groups will be analyzed. They were selected and divided by the beverage type and percentage as follows: beer, wine, high percentage liquors. As the majority of studies show, it’s important to underline the significance of not only the alcohol percentage implied, but mostly of the alcohol type. In favor of alcoholic beverages are only studies analyzing beverages containing low levels of alcohol, but abundant in other organic substances like resveratrol (in wine). Other studies considering other beverages, nonetheless of alcohol levels, diminishing effect on the cardiovascular (and other) systems is observed.    Material and methods: An analysis of scientific papers from Pubmed Google Scholar was performed, wchoch most accurately described the issue of diffrent type of alcohol bevereage on human health. The following keywords were used in search: alcohol, cardiovascular, wine, beer, spirits.  Purpose of the work: Aim of the following analysis is to systematize the knowledge based on latest research and findings about the impact of different alcohol beverages on cardiovascular system.