The aim of this study was to assess the prevalence of different serotypes of Salmonella spp. strains in poultry farming in N'Djamena and Doba towns. The strains were isolated on Hektoen agar medium, then identified through API 20E gallery, and serotyped by the direct agglutination technique on blade according to Kauffmann and White scheme. From a total of 1090 samples analyzed, 40 Salmonella strains corresponding to a prevalence of 3.67 % were identified, and regrouped into 11 serotypes: Salmonella Idikan (20%, n=8), Salmonella Anatum (15%, n=6), Salmonella Mbandaka (12.5%, n=5), Salmonella Limete, Salmonella Infantis, Salmonella Derby (10%, n=4), Salmonella Virchow (7.5%, n=3), Salmonella Gallinarum, Salmonella Choleraesuis (5%, n=2), Salmonella Paratyphi A, Salmonella Enteritidis (2.5%, n=1). Each sample taken apart contained at least one serovar, indicating that poultry farming in Chad is seriously affected by Salmonella. Moreover, Salmonella Paratyphi A and two strains of Salmonella Choleraesuis and peculiar respectively infecting man and pig were also isolated, probably due to fecal contamination of poultry food or water. These results reveal for the first time, a wide distribution of Salmonella spp. serovars in modern and traditional poultry farming sectors in N'Djamena and Doba in Chad.