Mycoplasma murisPCR New strain 16S rRNA sequences NIH MiceMycoplasma muris (M.M) is a small pathogenic bacterium that lives in the female mouse genital tract. Mycoplasma muris may have harmful effects on the reproductive health of female. This research was performed to optimize the detection of M. muris in NIH mice in the Department of Animal Breeding, Razi Vaccine and Research Institute, Iran. In this cross-sectional study, 29 vaginal samples of NIH mice were selected through simple random sampling. For detection of the mycoplasma, the vaginal tissue removal of samples was done. First, samples were crushed using mortar and pestle with PBS 1ml, then were cultured in the PPLO broth and incubated at 37°C for 24h, they were passed through 0.45 μm pore-size filters and inoculated into specific PPLO broth and agar media for 3-4 weeks. In the next section, the PCR test was used with primers of 16S rRNA gene of M. muris. From 29 tested samples, 17.24% samples were positive for M. muris by PCR method, while 35.93% cultures showed positive. The phylogenetic analysis indicated a new strain of M. muris. The results of culture and PCR methods displayed the contamination in NIH mice. Therefore, Therefore, more researches are needed regarding the presence of mycoplasma for treatment and clinical signs. Article Info

BackgroundAfter surgery and loss of anesthetic effect, postoperative pain can annoy the patient and affect patient satisfaction with treatment. This study was aimed at evaluating the effect of preoperative pentoxifylline (PTX) on postoperative pain and development of secondary hyperalgesia in patients undergoing laparoscopic appendectomy (LA).MethodsThis randomized, double‐blind, placebo‐controlled clinical trial study was conducted on 91 eligible subjects with acute appendicitis referred to Shahid Beheshti hospital of Sabzevar, Iran, in 2018. The intervention and control groups were administered with a single oral dose of PTX (10 mg/kg) and placebo an hour before surgery, respectively. Postoperative pain was measured within 24 hours after surgery using a VAS, and the area of secondary hyperalgesia was measured 24 hours after surgery using the Stubhaug et al. method.ResultsThe mean age of the subjects was 26.74 ± 9.99 years, and 57.14% were female. Pain intensity during rest was significantly greater in the control group as compared to the PTX group 24 hours after surgery (VAS scores 2.19 ± 0.49 and 3.13 ± 0.66, respectively; P < 0.001). Moreover, pain intensity during cough was substantially lower in the PTX group compared with the control group 24 hours after surgery (VAS scores 2.65 ± 1.90 and 4.10 ± 2.60, respectively; P = 0.003 in turn). The dynamic hyperalgesia was significantly greater in the control group as compared with the PTX group (3.80 ± 1.82 and 7.43 ± 2.38, respectively; P < 0.001).ConclusionsFindings suggest that oral administration of PTX 1 hour before surgery in patients undergoing LA can reduce postoperative pain in patients and prevent secondary hyperalgesia at a surgical site.

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Background and Aims: Psoriasis is a chronic inflammation of the skin caused by the proliferation of inappropriately differentiated horn cells, resulting in plaque psoriasis formation. It is not often fatal, and it may lead to physical disabilities and severe mental stress in which professional and social activities could be highly affected. It is generally accepted that the human leukocyte antigen (HLA)-CW06 allele has a significant role in the onset of this disease. Materials and Methods: This study determines the association between the HLA-CW06 allele and psoriasis. The present case-control study was conducted to evaluate the relation between HLA-CW06 and psoriasis in a population in Iran. This study was performed on 30 samples of patients with psoriasis. Results: The results of polymerase chain reaction- sequence-specific primers for the detection of HLA-CW06 showed positive amplification in 7 out of 30 psoriasis patients as compared to 4 among 30 controls. Conclusion: This study showed that due to different allele frequencies associated with psoriasis in different parts of the world, it seems that other genetic and epigenetic factors may be involved in this disease.