A total of 300 random eggs, representing 60 samples, (30 from baladi hens, and 30 from poultry farms) were collected from different markets, poultry farms, groceries and supermarkets located in Assuit governorate, Egypt. Each egg sample composed of 5 eggs and these samples were examined for the incidence of Helicobacter species using Helicobacter pylori special peptone agar (HPSPA). The results revealed that the incidence of Helicobacter species from examined baladi hen's egg shells was 10 (33.33%), poultry farms hen's egg shells was 9 (30%) and 1(3.33%) from examined baladi hen's egg content samples. H. pylori was isolated in a percentage of 5 (16.67%) from examined baladi hen's egg shells while from poultry farms hen's egg shells was 4 (13.33%). In addition, other Helicobacter species were isolated from the examined samples as H. cinaedi in a percentage of (6.67%) from examined baladi hen's egg shells and (10%) from examined poultry farms hen's egg shells. H. felis was isolated in a percentage of (6.67%) from both examined baladi and poultry farms hen's egg shells samples and H. pullorum was isolated in a percentage of (3.33%) from examined baladi hen's egg shells. Also, it was isolated from examined baladi hen's egg contents in a percentage of (3.33%). Identification of ureC gene for H. pylori isolated from both baladi hen's egg shells and poultry farms hen's egg shells by using polymerase chain reaction (PCR) revealed that 8 from 9 samples were positive for H. pylori by using (PCR) in a percentage of (88.89%).