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Aim:The study was conducted to evaluate the effect of ultrasonic irrigation activation on the depth of penetration of bioceramic sealer (CeraSeal) and Epoxy resin sealer (Adseal) into the dentinal tubules with cold lateral compaction obturation technique.Materials and Methods: 56 extracted upper central incisors were divided randomly into four experimental groups (each group, n = 14) based on sealer type and irrigation method. Obturation of teeth was performed using lateral compaction technique. Scanning electron microscope (SEM) was used to evaluate sealer penetration at 1600x magnification.Results: CeraSeal has significantly higher tubular penetration with ultrasonic activation in all sections. There was significant difference between all sections among each group as coronal was significantly the highest while apical was significantly the lowest. Conclusion:According to the limitations of the present study, irrigation activation is an essential step in canal preparation for better smear layer removal that gives deeper sealer penetration inside the dentinal tubules leading to a higher sealing ability of obturation. Bioceramic sealers have higher sealing ability than Epoxy resin sealers.

Objective:The purpose of this study is to compare the cytotoxicity of bioceramic endodontic sealers (Well-Root ST "Vericom in Gangwon-Do, Korea") with epoxy resin-based endodontic sealers (AH + sealer "Dentsply Sirona, Germany"). Methods:In sterile test tubes, the tested sealers were serially diluted twice. In order to evaluate the proper concentration where fibroblast cells would survive, extraction media were diluted many times utilizing MEM-E Medium (Eagle's minimal essential medium). The cytotoxic effect of concentrations used in the present study (0%, 6.25%, 12.5%, 25%, 50%, and 100%) was assessed by MTT essay after 24 hours using a human fibroblast cell line. Statistical analysis was performed by using One Way ANOVA test, followed by Tukey`s Post Hoc test for multiple comparisons.Results: All concentrations, with the exception of 12.5% and 25%, did not significantly differ between the two groups. Because group I (AH Plus sealer) had a lower proportion of viable cells than group II (Well-Root ST sealer), it had a larger cytotoxic impact. The concentrations that caused a significant difference between the two groups with a P value of 0.001 were 12.5% and 25%. Conclusion:Evaluation of the calcium silicate-based sealer (Well-Root ST) cytotoxicity showed superior biological behavior and higher cytocompatibility compared to epoxy resin-based endodontic sealer (AH plus).