Karyomorphological features of Aspidistra longifolia and A. subrotata from Thailand were here reported. The karyotype of A. longifolia is analyzed and confirmed as 2n= 38= 24m+2sm+12st in Doi Tung, Phu Hin Rong Kla National Park, and Nam Nao National Park. The tetraploid population of A. subrotata has been found in Phu Luang Wildlife Sanctuary, as the second tetraploid population reported for this species so far and the westernmost known record.
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