<p>Coagulase positive <em>Staphylococcus aureus</em> is the most frequently bacterial pathogen causing clinical mastitis in cattle. In this work 120 milk samples were collected from cattle suffering from clinical mastitis. The result revealed that S. aureus was 35 isolates (29.16%) and other staphylococci were 13 isolates (10.84%). The application of PCR was effectively in detection of Coa. SpA, mecA and blaZ genes of <em>S. aureus</em> by amplification at a single amplicon at 630 bp, 226 bp, 310 bp and 173bp, respectively. These results concluded that the detection of Coa and SpA genes proposed as good method for typing of <em>S. aureus</em> isolates as well as detection of antibiotics resistance mecA and blaZ genes helping effective control of staphylococcal mastitis.</p>