The study tested a new feeding protocol to find out the best program using cheap and available products to enhancement the mass culture and the nutritional value of B. plicatilis. The feeding regime was six artificial treatments formulated from dried yeast (Saccharomyces cervisiae) with sucrose sugar and yeast with molasses in different concentrations. Additionally, a live Cyclotella sp. had been applied as supplementary food to each treatment and as independent treatment (control). B. plicatilis was cultured in small scale to find the best food regime and technique to apply it in the large one. B. plicatilis attained its highest density in the small scale culture (370 Ind.ml1 ) with T1 (30 % yeast, 70 % sugar and Cyclotella sp.) at the 12th day, while the highest population growth rate (PGR) (0.65) was calculated at the 9th day. According to the analysis of variance (ANOVA) and PGR, T1 had been applied in the large scale culture. B. plicatilis samples were composed of 51.6% total protein with 16 amino acids and 33.01% total lipid with 19 identified fatty acids. The study concluded that the treatment (T1) is a suitable diet for enrichment the mass culture and the nutritional value of B. plicatilis.

Aquatic environment is a vast reservoir of bioactive natural products, many of which exhibit structural and chemical properties not found in terrestrial natural products. However, studies on aquatic medicines have included a very small proportion of aquatic organisms. Copepoda is one of the most diverse groups on earth, yet there are no studies to test omnivores Copepod extract as an antimicrobial and anticancer agent. Therefore, this study aims to improve the sustainable mass production of the copepod Acanthocyclops trojani to evaluate its crude extract for antimicrobial and anticancer activity. GC-MS analysis of A. trajani extract detected 8 bioactive compounds known for their medicinal uses in several previous studies. The A. trajani crude extract showed activity against bacterial strain; S. aureus, E. coli, E. faecali, K. pneumonia, S. lutea and S. typhi with zones of inhibition of 14, 12, 8, 12, 17 and 12 mm, respectively. In addition, A. trajani crude extract showed moderate growth inhibitory activity against HepG2, A549, HCT and MCF7 cancer cell lines (IC50 = 63. 064, 18.377, 46.905 and 21.736, respectively) compared to the activity of staurosporine (control). While the normal cells (THLE2, WI38, FHC and MCF10a) were more sensitive to staurosporine than the A. trajani extract. Based on the Cytotoxicity Selective Index (SI) values, the extract showed a selective effect on the lung and breast cancer cells (A549 and MCF7) (SI = 3.07 and 3.1, respectively). Therefore, A. trajani extract offers the potential for further exploitation in the discovery of a novel antibacterial agent and against lung and breast cancer.

No abstract

Aquatic environment is a vast reservoir of bioactive natural products, many of which exhibit structural and chemical properties not found in terrestrial natural products. However, studies on marine medicines have included a very small proportion of aquatic organisms. Copepoda is one of the most diverse groups on earth, yet there are no studies to test Copepoda extract as an anti-cancer agent. Therefore, this study aims to improve the sustainable mass production of the copepod Acanthocyclops trojani to evaluate its crude extract for antimicrobial and anticancer activity. GC-MS analysis of A. trajani extract detected 8 bioactive compounds known for their medicinal uses in several previous studies. The A. trajani crude extract showed activity against bacterial strains; S. aureus, E. coli, E. faecali, K. pneumonia, S. lutea and S. typhi with zones of inhibition of 14, 12, 8, 12, 17 and 12 mm, respectively. In addition, A. trajani crude extract showed moderate growth inhibitory activity against HepG2, A549, HCT and MCF7 cancer cell lines (IC50 = 63.064, 18.377, 46.905 and 21.736, respectively) compared to the activity of staurosporine (control). While the normal cells (THLE2, WI38, FHC and MCF10a) were more sensitive to staurosporine than the A. trajani extract. Based on the Cytotoxicity Selective Index (SI) values, the extract showed a selective effect on the lung and breast cancer cells (A549 and MCF7) (SI = 3.07 and 3.1, respectively). Therefore, A. trajani extract offers the potential for further exploitation in the discovery of a novel antibacterial agent against lung and breast cancer.