The effect of lime and fertilizer application, as the management of soil acidity, on the growth and yield of rice cv. BRRI dhan50 was investigated during Aman rice season at the Agronomy Field Laboratory of Sylhet Agricultural University, Bangladesh. The experiment was consisted of two factors namely lime and fertilizer. There were four levels of lime (0, 0.50, 1.00, and 1.50 t ha–1 of CaCO3.MgCO3) and three levels of fertilizers (control, FYM @ 10 t ha–1, and chemical fertilizer @ 100-30-42-4-3-0.4 kg ha–1 of N-P-K-Ca-S-Zn). The experiment was laid out in a randomized complete block design with three replications where the unit plot size was 4.0 m x 2.5 m. Growth parameters, yield components and yield of BRRIdhan 50 rice increased with increasing lime rate in association of fertilizer in acidic soil. The highest grain yield (2.90 t ha–1) was recorded from the application of 1.50 t ha–1 lime and the lowest (2.06 t ha–1) was from control (0t ha–1), irrespective of fertilizer. On the other hand, the best effect of fertilizers on grain yield (3.08 t ha–1) was found with the application of FYM @ 10 t ha–1 and the lowest yield (1.59 t ha–1) was in control. The treatment combination of lime 1.50 t ha–1 and FYM (@ 10 t ha–1 produced the highest grain yield (3.60 t ha–1), which was followed by treatment combination of lime 1.50 t ha–1 and chemical fertilizer @ 100-30-42-4-3-0.4 kg ha–1 of N-P-K-Ca-S-Zn (3.28 t ha–1). Additionally, application of lime and FYM improved the soil fertility and properties of acidic soil for crop production by increasing the pH, organic matter and availability of some essential nutrients. From the study, it was indicated that both FYM and lime could affect to enhance the grain yield of rice in acidic soil. J. Bangladesh Agril. Univ. 16(3): 357–365, December 2018

The fusarium yellows resistance (YR) gene FocBo1 was previously identified and the DNA markers were developed to assist the breeding of YR cultivars in Brassica oleracea . However, the further analysis revealed discrepancies between the phenotypes and the genotypes predicted by those DNA markers in cabbage commercial cultivars. Since this discrepancy seemed to be due to unknown susceptible alleles of focbo1 , we sequenced the gene in 19 accessions to determine the sequence variations between alleles and found that there were two resistant FocBo1 alleles and six susceptible alleles in the investigated population. The newly designed PCR markers detected three mutations in the susceptible alleles that generate premature termination codons. These were shown to accurately distinguish resistant and susceptible alleles in more than 200 accessions of B. oleracea inbred lines and cultivars. The study revealed that the locus is represented by 37.2% resistant and 62.8% susceptible alleles within seventy-eight commercial cultivars. Structural analysis of the gene revealed that a part of the allelic variation comes from intragenic recombination between alleles. Our results enable a more precise prediction of the phenotype by marker assisted selection, promoting the production of YR cultivars in B. oleracea .

QTL mapping for tuberous stem formation of kohlrabi (Brassica oleracea var. gongylodes L.