Primary angiosarcomas arising from the alimentary tract are rare and only a few cases have been reported in the literature. We report a case of an angiosarcoma of the sigmoid colon with intraperitoneal bleeding but not rectal bleeding. A 21-year-old female patient received a laparotomy and a mass lesion over the sigmoid colon was found with active bleeding. A sigmoid colectomy was performed as a curative resection. Grossly, the sigmoid colon contained a kidney shaped, hemorrhagic tumour from the submucosal layer extension to the antimesenteric side. Intraluminally, the mucosa of the colon was intact. Microscopic examination revealed a high grade angiosarcoma composed of fascicles of spindle cells and solid sheets of epithelioid cells. Immunohistochemical stains revealed a positive result for CD31 and the endothelial nature of the malignancy was confirmed. Smooth muscle antigens, desmins, cytokeratins AE1/AE3 and CD117 were all negative. The patient is still alive without evidence of recurrence or metastasis at a three-year follow-up appointment. Owing to the availability of immunohistochemical studies, some atypical sarcomas would now be correctly classified as angiosarcomas. Since no optimal adjuvant treatment is effective, curative surgical excision is still the best choice of treatment.Ann R Coll Surg Engl 2011; 93: e91-e93 doi 10.1308/147870811X591017 An angiosarcoma, which usually affects adults and can develop from the endothelium of the lymphatics (lymphangiosarcoma) or blood vessels (haemangiosarcoma), is a rare tumour among soft tissue sarcomas. The incidence is low and represents less than 1% of all sarcomas.1 They occur most commonly in the skin and soft tissue and less commonly in the breast, liver, bone and spleen. Gastrointestinal angiosarcomas are very rare, with only a few case reports in the literature.In 2004 Brown et al summarised the 13 reported cases of colorectal angiosarcomas in the literature.2 Of those cases, the most diagnostic symptom was rectal bleeding, caused by the inner invasion of the tumour. In this paper, we introduce another case of a primary colonic angiosarcoma with an atypical presentation of intraperitoneal bleeding but not rectal bleeding. We also discuss its gross appearance, histopathological features, immunohistochemical profiles, management and prognosis. case historyA previously well 21-year-old female patient presented to the gynaecology department having had low abdominal pain for several days. The abdominal ultrasound revealed a huge tumour mass over the left lower abdomen with fluid accumulation in the pelvis. The haemoglobin level was 10.1mg/dl on the day of admission. A diagnostic laparoscopy was arranged due to the suspicion of a left ovarian tumour with bleeding.A huge tumour mass over the sigmoid colon and 500ml bloody ascites were noted at laparoscopy. Both ovaries were normal. Because of uncontrolled bleeding from the lesion of the sigmoid colon, a sigmoid colectomy was indicated and the operation was converted to an open laparotomy. After dissecti...

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Purpose: Target therapy and immunotherapy are advancements in the treatment of melanoma; unfortunately, a subset of patients does not benefit or produces drug resistance. Therefore, an alternative strategy should be investigated to further improve clinical benefits. Macrophages are the abundant immune cells related to the progression of melanoma and resistance to drugs. In this study, we explored the novel roles of miR-567 in the progression of melanoma and effects of macrophages. Methods: miR-567 expression in nevus and melanoma tissues was analyzed by in situ hybridization. miR-567 was transfected into melanoma cells to investigate its biological effects through functional assays such as proliferation, colony formation, soft agar, and migration assays. In addition, miR-567-related signal transduction pathways in the melanoma cells were studied by western blot and next-generation sequencing analyses. Different phenotypes of macrophages polarized from THP-1 monocyte cells were used as in vitro model to investigate the effects of miR-567 on macrophages. Results: Our results showed that miR-567 expression levels were decreased in melanoma cells compared to melanocyte cells. Consistently, expression of miR-567 was 0.403-fold lower in melanoma tissues (n=118) as compared to nevus tissues (n=40). In addition, the receiver operating characteristic presented that the area under the curve value of miR-567 is 0.9495. Importantly, higher expression of miR-567 enhanced the overall survival of melanoma patients. Overexpression of miR-567 significantly reduced proliferation, survival, anchorage-independent growth, migratory, and invasive abilities of melanoma cells and BRAF-inhibitor resistant cells. Furthermore, our results demonstrated that IGF1R, E2F1, and Cyclin B2 are direct targets of miR-567 and knockdown of these genes attenuated proliferation and survival of melanoma cells. Interestingly, introduction of miR-567 downregulated MAPK/ERK and PI3K/AKT pathways in melanoma cells and regulated multiple pathways related to immune response. Of note, overexpression of miR-567 reduced the promoting melanoma growth induced by M2 or melanoma-associated macrophages, indicating the involvement of miR-567 in regulation of macrophages. Conclusion: miR-567 could be served not only as biomarkers but also as potential molecular target for prevention of melanoma progression. Citation Format: Mai-Huong Thi Nguyen, Chen-Huan Lin, Yu-Chi Huang, Mu-Shiun Tsai, Ming-Hong Chen, Azusa Miyashita, Satoshi Fukushima, Nianhan Ma. miR-567 modulates the progression of melanoma cells and functions of macrophages. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 3753.

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