Gold nanoparticles (AuNPs) are a highly versatile compound to be used in sensing components due to their unique physicochemical and optical properties. Due to its flexibility for surface conjugation, glutathione disulfide-linked gold nanoparticle (AuNP-GSSG) was produced in this study to assess its sensing performance as a colorimetric sensor for human chorionic gonadotropin (HCG). Spectroscopic characterization of AuNP-GSSG confirmed the conjugation of GSSG onto the surface of AuNPs. AuNP-GSSG also showed high sensory performance with good linearity and low detection limit. However, due to the high percentage of interference, usage of AuNP-GSSG as an HCG sensor requires further pre-treatment step to reduce the interference and increase the sensor accuracy.

Human chorionic gonadotropin (HCG) has been amongst of essential part of medical diagnostics in gynaecology and oncology since its discovery. Application of gold nanoparticles in detecting HCG is due to gold nanoparticles possess extraordinary optical and physical properties, making them invaluable in various prominent aspects, including its synthesis, stabilization, and functionalization as a sensor. In addition, microfluidic analytical device, a platform for point-of-care testing and numerous applications in various field, can be applied for detection of HCG using diverse types of detection methods. From the literatures, combination of gold nanoparticles and microfluidic analytical device can be assembled to obtain a rapid, simple and user-friendly analytical site for detection of HCG.