The current study aimed to know the effect of rutin at a dose of (30 mg/kg of body weight) and (60 mg/kg of body weight) on heart tissues in two groups of normal rats and two groups of rats exposed to oxidative stress with 1% hydrogen peroxide for the duration of the experiment., as well as the effects of rutin on the percentage of some antioxidants such as glutathione GSH, and the percentage of malonaldehyde (MDA) in the serum. (48) male rats were used, they were divided into six groups.: the first group (control) included eight males, and the second group hydrogen peroxide H2O2 1%, H2O2, and rutin at a concentration of 60 mg/kg, H2O2 with a concentration of rutin 30 mg/kg, and with a concentration of rutin at a concentration of 60 mg/kg and with a concentration of 30 mg/kg of rutin. All groups dosed daily for one month, after taking blood samples, sacrificing the animals, and making tissue sections of the heart under study. The results revealed that Rutin increased the GSH glutathione ratio in the groups in which oxidative stress was induced by hydrogen peroxide by 1% compared to the control group treated with hydrogen peroxide only. While rutin reduced MDA in the same groups compared to the control group that was treated with hydrogen peroxide by only 1%. At (P≤ 0.05) The histological study of the heart of mice treated with Rutin revealed a high cure rate, as Rutin showed protection of the heart tissue from the toxicity caused by hydrogen peroxide. While the tissue sections of the positive control group that were treated with 1% hydrogen peroxide showed clear tissue degeneration with hemorrhage and necrosis. The histological results of two groups treated with concentrations of (30 mg/kg of body weight) and (60 mg/kg of body weight) showed no toxic effects of rutin compared with the negative control group. Keywords: Rutin, heart, GSH, MDA

The current study was designed to investigate the effect of Rutin on some physiological parameters related to the heart in male white rats exposed to oxidative stress with hydrogen peroxide. The study included 48 adult male white rats, their weights ranged from (220-280) g, and their ages ranged between (8-12) weeks. The rats were randomly distributed into six groups of 8 rats for each group, and as follows: The first group, group 1, was considered a negative control group G1 that was supplied with water and the diet for the duration of the experiment which amounted to 30 days and the second group, group 2, was considered a positive control group that was given drinking water containing hydrogen peroxide at a concentration of 1% by means of special drinking bottles and the diet for 30 days, the third group G3 was orally dosed with Rutin at a concentration of 60 mg/kg of body weight in addition to drinking water containing hydrogen peroxide at a concentration of 1% for 30 days . The fourth group G4 also dosed orally with a solution of Rutin at a concentration of 30 mg/kg bw in addition to drinking water containing hydrogen peroxide at a concentration of 1% for 30 days. fifth group G5 dosed orally with a Rutin only at a concentration of 30 mg / kg bw for 30 days. the sixth group G6 was also dosed orally with Rutin only at a concentration of 30 mg / kg bw for30 days. After the end of the experiment, the animals were anesthetized then blood was drawn from the heart directly for the purpose of obtaining serum to measure the level of Troponin, LDH, CK-MB and Myoglobin. The results showed a significant increase (p < 0.05) in the level of Troponin, LDH and Ck-MB in the positive group compared to the negative control group, while a significant decrease in the levels of these enzymes was observed in the third and fourth groups compared to the positive control group, while in the two groups The fifth and sixth recorded a significant decrease compared to the positive control group. After obtaining these results, it became clear that Rutin compound has a positive effect in preventing oxidative stress and heart muscle damage. Keywords: Rutin, hydrogen peroxide, Rats.