Antioxidants can be found naturally in plants, animals, and microbes, or they can be produced chemically. The selection of plants to find the new bioactive compounds are carried out through ethnobotany and chemotaxonomic approaches. One plant that can be developed as an antioxidant is parang romang (Boehmeria virgata). This study aimed to identify an antioxidant activity on the roots, stems, leaves, and flowers of parang romang plant qualitatively and quantitatively. The parang romang plant used was obtained from Malino Regency, South Sulawesi. The roots, stems, leaves, and flowers were extracted using the reflux method and then concentrated using a rotary evaporator. Each part was analyzed for its chemical content and activity qualitatively and quantitatively. Qualitative analysis was carried out with sitroborat, folin ciocalteu, DPPH, FRAP, and CUPRAC reagen, while quantitative analysis was carried out by spectrophotometry using the DPPH method. The roots, stems, leaves, and flowers were detected to contain phenolic, and flavonoid compounds and have antioxidant activity based on qualitative analysis. The antioxidant activity of parts of the parang romang plant is classified as strong to very strong, with an AAI value of 1.5 - 4.54. All parts of the plant tested have antioxidant activity.
The bajakah (Spatholobus littoralis Hassk.) is one of the typical plants from Kalimantan Island, which has potential as a drug, one of which using as an antioxidant is due to the secondary metabolites contained therein. The aims of the research are to estimate the total phenolic content, total flavonoid content, and antioxidant capacity of the bajakah by different methods. Extraction was carried out by reflux method using a solvent gradient of n-hexane, ethyl acetate, and ethanol 96%. Phenols and flavonoid content were calculated using spectroscopy. Antioxidant capacity was measured against ascorbic acid and Trolox using the CUPRAC, DPPH, and FRAP methods and reported as mg Trolox equivalent (TEAC) and ascorbic acid equivalent (AEAC) per gram extract. The ethanol extract showed the highest antioxidant capacity for the CUPRAC and FRAP methods, the n-hexane extract for the DPPH method on both varieties of bajakah tampala. Each method provides significantly different antioxidant capacity values. The CUPRAC and FRAP methods found the highest antioxidant capacity in the white variety of bajakah ethanol extract. Meanwhile, in the DPPH method, saw the highest antioxidant capacity in the red variety of n-hexane bajakah extract. DPPH and CUPRAC give a positive correlation with TPC, where CUPRAC assay produced higher values than FRAP and DPPH assays.
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