Antibiotic resistance against present antibiotics is rising at an alarming rate with need for discovery of advanced methods to treat infections caused by resistant pathogens. Silver nanoparticles are known to exhibit satisfactory antibacterial and antibiofilm activity against different pathogens. In the present study, the AgNPs were synthesized chemically and characterized by UV-Visible spectroscopy, scanning electron microscopy, and X-ray diffraction. Antibacterial activity against MDR K. pneumoniae strains was evaluated by agar diffusion and broth microdilution assay. Cellular protein leakage was determined by the Bradford assay. The effect of AgNPs on production on extracellular polymeric substances was evaluated. Biofilm formation was assessed by tube method qualitatively and quantitatively by the microtiter plate assay. The cytotoxic potential of AgNPs on HeLa cell lines was also determined. AgNPs exhibited an MIC of 62.5 and 125 μg/ml, while their MBC is 250 and 500 μg/ml. The production of extracellular polymeric substance decreased after AgNP treatment while cellular protein leakage increased due to higher rates of cellular membrane disruption by AgNPs. The percentage biofilm inhibition was evaluated to be 64% for K. pneumoniae strain MF953600 and 86% for MF953599 at AgNP concentration of 100 μg/ml. AgNPs were evaluated to be minimally cytotoxic and safe at concentrations of 15-120 μg/ml. The data evaluated by this study provided evidence of AgNPs being safe antibacterial and antibiofilm compounds against MDR K. pneumoniae.

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The study was planned to determine the comparative efficacy of leaf extract of Azadirachta indica, Cichorium intybus and Moringa oleifera, on haematology, intestinal histomorphology and nutrient digestibility in broilers. Day old broiler chicks (n=180) purchased from a commercial hatchery were reared in a group for one week (adaptation period). At day 8, these birds were individually weighed and 120 birds of middle weight range were randomly selected and distributed into 12 replicates (10 chicks/replicate). These replicates were further allotted to four treatment groups designated as A, B, C and D. Group A was offered water without any supplementation and served as a control. Whereas, group B, C and D were offered water supplemented with leaf extract of Azadirachta indica (4%), Cichorium intybus (2%) and Moringa oleifera (6%), @ 50 ml/l, @ 10 ml/l and @ 30 ml/l, respectively. Results of the study revealed that supplementation of Azadirachta indica leaf extract showed better nutrient digestibility of crude protein and ether extract as compared to that of control. However, digestibility of crude fiber due to the treatments remained unaffected. Stabilization of blood metabolites resulted in improved intestinal histo-morphology. The birds using Azadirachta indica fetched the highest profit as compare to the other treatment groups. Therefore, use of Azadirachta indica leaf extracts in broiler is recommended as inexpensive and efficient growth promoting agent without residual effects like antibiotic growth promoter.

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