Role of ICT and knowledge among professionals of organizations directly affects the quality of output. In this study the levels of aspect for utilization of ICT resource at workplace among professionals from different organizations in Karachi have assessed. These organizations belong from different sectors including public and private. Data was obtained from the employees of different organizations through online questionnaire. The Survey was conducted from October 15 – December 4, 2015. Questions were asked about its effect on their lives and work. A Sample of 24 employees was responded to the questionnaire and was completed for analysis. A total of 24 employees were assessed for frequency distribution of ICT Knowledge factors and descriptive analysis test was used to see the association of online system, computer exposes organization works, usage of the latest technology, internet, E-commerce and different statistical software's related to ICT knowledge. The study has few recommendations on the basis of data analysis and findings. Although the knowledge of ICT is good but some professionals and organizations are not utilizing the ICT resources completely to achieve the better results. They should improve and enhance their staff skills and knowledge through different trainings and upgrade their systems for fulfillment of their requirements and productive outputs. In addition, the Government of Pakistan has established the Ministry of Information Technology and Telecommunication Division in March 2000. The Ministry of IT is building Pakistan's IT competency to meet the challenges of 21st Century. Similarly, IT Departments are created in all Provinces for taking initiative for development of IT as per requirement of 21st century and promotion of IT education and development. The local institutions and universities do not seem to produce highly skilled people in the field of IT, especially, in certain required fields, such as programming and hardware engineering. The strength of IT education and proper trainings in the relevant field are essential. However, it seems that the focus of our educations institutions is on the quantity of the number of students being awarded degrees each year rather than imparting quality education. Thus, the challenges of the 21st century cannot, undoubtedly, be met with the present human resource. We strongly suggest taking serious measures and reforms in the education system, support IT industry in order to create a good job market, and attract investors from around the globe by providing subsidized business.