The Experiments were setup in three locations, Magura (23°3´ N 89°2´ E), Rangpur (25°7´ N 89°3´ E) and Mymensingh (24°8´ N 90°0´ E), Bangladesh to know the effect of two bacterial strains and 3 nitrogen doses on nodulation, yield and biomass production. The experimental material consists of one lentil popular variety viz., Binamasur-5 which was inoculated with two promising rhizobial starins/isolates namely LB-21 and LB-40 and three levels of nitrogen viz., 25, 50 and 75 kg N ha-1. The inoculant treatments were un-inoculated control (I0), LB-21 (I1), LB-40 (I2) and mixture of LB-21 and LB-40 (I1+I2). Results indicated that nodule number, nodule weight, straw yield and seed yield at the three locations were greater in bacterial isolates treated plants than in control plants. Results further indicated that mixture of two bacterial isolates had no superior effect against any single bacterial isolate. The two isolates (LB-21 and LB-40) had almost similar greater effect on nodule number, nodule weight, physiological parameters and seed yield with being the highest in LB-40. Application of 25 kg N ha-1 under both bacterial isolates performed the best in seed yield. Application of nitrogen at the rate of 75 kg ha-1 had adverse effect on nodulation, growth and seed yield in lentil. Therefore, for getting higher seed yield in lentil, should apply N as basal dose at the rate of 25 kg N ha-1 and the bacterial isolate, LB-40 may be registered as biofertilizer after few more trials in the different lentil growing areas of Bangladesh.
The Experiment was performed under glass house condition (24°8´ N 90°0´ E) with eight rhizobial strains namely MBR-3, MBI-5, MBI-19, MBM-4, MBM-8, MBP-10, MBB-3 and MBJ-7 obtained from mungbean rhizosphere of different locations in Bangladesh and BINA MB-1 (a registered biofertilzer for mungbean) was used as standard check along with un-inoculated control to test their potentiality under glass house condition for mungbean. Result revealed that the higher plant growth, biochemical parameters, seed yield attributes and seed yield were recorded in three bacterial isolates viz., MBI-5, MBB-3 and biofertilizer, BINA MB-1 with being the highest in MBI-5. Therefore, the isolate MBI-5 may be used as commercial biofertilizer after few more trials in the different mungbean growing areas of Bangladesh Res. Agric., Livest. Fish.7(2): 191-197, August 2020
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