The objective of this study was to select and identify the sample and to perform extraction of Sterculia lychnophora and to check the presence of phytochemicals by preliminary test along with HPTLC method to be used for qualitative analysis. In present investigation primary screening of phytochemical and qualitative analysis of secondary metabolites like tannins, saponins, phenols, terpenoids, flavonoids, alkaloids and lipids were carried out by HPTLC method. Higher percentage of lipid, tannins and phenol, moderate of flavonoids, saponins and terpenoids while fewer peaks of alkaloids were observed. The present work signifies the use of HPTLC fingerprint for determining the identity, purity of drugs and also for developing standards
Background:is an important aromatic herb. Since the ancient time plant is being used as Pogostemon benghalensis medicines, agrochemicals and pharmaceutical by large number of tribal and rural people. Almost all parts of the plant are helpful to treat different kinds of aliments; very few of these claims have been reported and scientifically studied. Objectives: The present study focused on the phytochemical analysis, antioxidant activity, RBC haemolysis and antimicrobial activity.Aqueous and methanol extracts of leaves of , used for Materials and methods:P. benghalesis Phytochemical analysis revealed major components such as flavonoids, saponins, tannins, glycosides, alkaloids and phenolic compounds present in both PBE.Results of the study showed that Results and conclusion: P. benghalensis extract showed significant antioxidant and RBC haemolysis activity. Antibacterial analysis of showed P. benghalensis moderate to high activity against Staphylococcus aureus, Escherichia coli, Salmonella typhi, Streptococcus pyogenes and respectively. Therefore, the phytoconstituents in this plant are found to be with high Klebsiella pneumonia, medicinal value and can be explored for further studies. There seems enormous potential and scope for future research and further pharmacological investigation on . Pogostemon benghalensis
Antagonistic activity of marine bacteria against common human pathogens was studied. Marine animal surface bacterial flora was isolated from selected animal Scoliodon sarrakwah (Shark), Mandeli (Golden anchovies) Colia dussumerii, Promfret (Silver) Pampus argenteus and prawns Penaeus indicus collected from local fishermen. The sample bacteria were isolated by serial dilution followed by spread plate method using Zobell's marine agar (ZMA). About 11 bacterial isolates were selected and screened for antimicrobial activity through agar well diffusion assay. Among 11 isolates, 7 isolates exerted an inhibitory effect against human pathogenic organisms (E. coli, S. aureus, Klebsiella and Candida sp.). The isolates were then investigated for antibiotic production by cross species signal transfer mediated induction, 2 isolates (Sh-2 and PJR-3) exhibited enhanced activity against human pathogenic organisms (E. coli, S. aureus and Candida sp.). These 2 isolates were characterized phenotypically by morphological techniques (which revealed the strains as gram-positive and gram negative respectively) and physiologically by conventional tests.
is a unicellular organism, widely distributed in the freshwater environment, where heavy metals are common contaminants. are very abundant group in aquatic ecosystems, which makes them effective biological indicators of water pollutants. The aim of this study was to determine the ability of to survive in various concentrations of three different heavy metals: Ferrous Sulphate (FeSO), Mercuric Chloride (HgCl) and Zinc Chloride (ZnCl). The ciliates were incubated in solutions with 0.3 to 1.5 ppm of FeSO, 0.01 to 0.05ppm of HgCl and 0.10 to 0.95 ppm of ZnCl. at room temperature. Using compound microscope, microscopic observations of cell divisions rate was conducted after 24, 48, 72 and 120 hours of incubation in the tested solutions and was compared to the control sample. Microscopic observations revealed that all the concentrations of HgCl were lethal for all samples except sample no. 3 while FeSO and ZnCl were found to be growth promoter for of all 10 samples.
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