aging is an inevitable biological complex process. It involves the gradual loss of cellular vitality due to accumulative damage to cellular macromolecules by reactive oxygen species (ROS). These ROS are highly implicated in health, disease, and lifespan. The biochemical pathways involved in the aging process are highly influenced by both exogenous (environmental factors) and endogenous stress factors. These cellular processes are the same in most organisms including the fruit fly, nematode, yeast, mammalian cell line, and rodents. These model organisms have been extensively used in the screening of potent antioxidant botanicals for anti-aging bioactivity. Moringa oleifera and Ananas sativa are great sources of health-promoting nutrients and antioxidants, however, their anti-aging impact is still an evolving area of research interest. Therefore, this review focused on their anti-aging mode of action and some other anti-aging nutriceuticals in different model organisms including the fruit fly. Practical applications Staying forever young and healthy is everyone's right. Aside from genetic trait, healthy feeding is peculiar to the world's longest-living people. Ananas sativa (pineapple) and Moringa oleifera leaves are highly valued fruit and herb with nourishing, antioxidant, and medicinal properties. Their extract exhibit antioxidant, anticancer, anti-inflammatory, and anti-aging activities. The ancient Greeks, Egyptians, and Romans used Moringa seed oil for cosmetics and perfumes. Moringa tea leaves is consumed for its nutritive and medicinal value. Its antioxidant potency endorses its use for anti-aging and other health-promoting purposes. The bioactive compound in pineapple, bromelain, promotes wound healing and it is a component of postsurgical applications due to its anti-inflammatory property. Consumption of Ananas fruit provides the recommended daily allowance of vitamin C, a potent antioxidant. To identify new antiaging bioactive compounds of therapeutic importance, and understanding the mode of action of these nutriceuticals will contribute to new anti-aging research prospects.

Despite the nutritional value of Theobroma cacao seed (cocoa), the antiproliferative activity is yet to be fully elucidated. Therefore, the current study investigated the antioxidant and potential anticancer activities of the seed extract. The in‐vitro free radical scavenging activity was evaluated via DPPH, nitric oxide, lipid peroxidation, and reducing power activity. The effect of the cocoa extract on mitotic cell division was determined using Allium cepa assay. The phytochemical screening of the seed extract revealed the presence of cardiac glycoside, phenol, tannin, steroid, terpinoid, alkaloid, saponin, and flavonoid at the following concentrations; 31.21 ± 0.57, 29.16 ± 0.43, 18.51 ± 0.06, 9.57 ± 0.08, 23.77 ± 0.08, 6.58 ± 0.04, 28.65 ± 0.13, and 22.16 ± 0.18 mg/100 g, respectively. The A. cepa assay revealed a significant influence of the extract on mitotic cell division in a concentration dependent manner, the antiproliferative effect suggests a potential anticancer property. Practical applications The Cocoa plant has been of great nutritional benefit over the years. The seeds (Theobroma cacao) is used for making varieties of chocolate food beverages, chocolate candies, confectioneries, and food thickening, however, the anticancer activity is still an area of deliberation. Theobroma cacao seed extract scavenged diphenyl‐2‐picrylhydrazyl radical, nitric oxide, lipid peroxidation at 50.03 ± 1.48% at a concentration of 60 µg/ml, 50.84 ± 0.88% at 40 µg/ml, and 50.79 ± 0.37% at 40 µg/ml, respectively. The reducing power activity was 0.329 ± 0.001 per 100 µg/ml. The Gas Chromatography Mass Spectroscopy analysis showed the most abundant compound to be caffeine (98.09%). Mild quantities of hexa‐decanoic acid, methyl extract were also detected. The hydro‐alcohol extract of Theobroma cacao seeds exhibited high antioxidant and antimitotic activities, suggesting a possible cytotoxic potential against abnormal cell growth, such as cancer. This study substantiates its ethno‐medicinal use in the prevention and control of cancer.

Ethno-pharmaceutical products have received a lot of international attention in the scientific community in the management of diabetes mellitus (DM). In this study we determined the anti-diabetic and high dosage effects of Bidens pliosa in type 1 DM (T1DM). Methodology: Thirty rats were divided into six groups and subgrouped into the extract and non extract treatment groups. The extract treated group was subdivided into three groups which received 200 mg/kg, 400 mg/ kg and 800 mg/kg dosage treatments respectively. The blood glucose levels were monitored using a standard glucometer for one month, and biochemical analysis of the two liver function enzymes; Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) were carried out at the Institute of Biomedical Research (IBR-KIU-WC) at the end of week IV. The study revealed that Bidens pilosa maintained hypoglycemia for a period of two weeks and this status was lost in subsequent weeks. T1DM rats treated with a dosage of 200 mg/kg showed a better recovery (355.25-164.5 mg/dl) of the glucose levels, followed by those that were being treated at 400 mg/kg. The AST and ALT enzymes in blood varied with a mean ± SEM (33.72 ± 32.32 to −7.23 ± 12.61 IU and 22.98 ± 11.12 to 42 ± 38.2 IU, respectively) in both the glibencimide ® and in the 800 mg/ kg treatment groups in the study. High dosages of extract were associated (P = 0.049) with increased systemic enzyme leakage. In conclusion, tissue degeneration caused by high levels of the extract was accompanied by leakage of various enzymes (AST and ALT) into the blood, which could be a major etiological factor for the development of secondary systemic pathologies, thus potentially worsening the effects of an existing T1DM prognosis in human patients. The preliminary results indi-* Corresponding authors. Ajagun-Ogunleye et al. 147 cate that a dose of Bidens pilosa has an anti-diabetic effect for a limited initial duration before starting to cause systemic toxicological effects. It is highly recommended that further investigation into the cellular mechanisms and consequences of any therapy involving Bidens pilosa be carried out.

Aim: The epidemiology of coccidian parasites in HIV patients of sub-sahara Africa is poorly understood. This study aimed at determining the epidemiology of coccidian parasites and their associated risk factors. This was a cross sectional study carried out in Arua district in West Nile region of Northern Uganda for a period of five months. Materials and Methods: Participants in the study included HIV positive patients presenting with diarrhea. A total of 111 patients were included and classified into children, middle aged and adults. A structured questionnaire was administered, stool samples were obtained using sterile stool containers and laboratory analysis carried out using modified Ziehl-Neelsen technique (ZN). Ethical clearance was acquired and the consent of the patients was sought. Results and Discussion: Prevalence of Coccidian parasites among HIV patients was found to be 5.4% and Cryptosporidium parvum showed more prevalence than Isospora belli and Cyclospora cayatenensis i.e. (3.6%), (1.8%) and (0.0%) respectively. Most Cryptosporidium parvum infections occurred in children (13.6%) compared to adults (3.3%); with a significant relationship of (p = 0.02). The infection was higher in females (7.1%) than males (2.4%) (p = 0.19). The major risk factors associated with the disease were mainly consumption of contaminated and un-boiled water from taps and boreholes. HIV patients who took co-trimoxazole and drunk boiled water were shown to have a low prevalence of coccidian parasites of 1.9% and 2.6% respectively (p<0.05). This is because cotrimoxazole is a prophylactic drug for opportunistic infections and proper boiling of drinking water kills coccidian parasites. Conclusion and Recommendations: The study highlighted the importance and need to screen for coccidian parasites and emphasis on regular taking of prophylactic treatment as a way of controlling opportunistic infections in HIV patients. Future prevalence studies of Coccidia amongst healthy, HIV sero-negative children and adults of similar age groups in similar settings are recommended to ratify the relationship.

Background: Ageing is the major risk factor for most non-communicable diseases. It occurs as a result of free radical damage to macromolecules in the cell, coupled with environmental stress assaults. In the quest to identify new anti-ageing bioactive compounds from natural products, and despite the therapeutic values of Ananas sativa, its anti-ageing activity has not been fully elucidated. Therefore, the aim of this study was to screen the crude, methanol fruit extract of Ananas sativa (MEAS) and its fractions, for anti-ageing bioactivity in the fruit fly, Drosophila melanogaster w 1118 wild type flies. Materials and Methods: Flies reared on food supplemented with 5, 10, and 20mg/ml fruit pulp and juice extract were assayed for longevity, fertility and stress resistance according to established protocols. Phytochemical composition and scavenging activity of extract on 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) were equally evaluated. The crude extract anti-ageing activity was assayed through a successive bioassay-guided fractionation approach, with different extraction solvents; n-Hexane, Chloroform, Ethyl acetate and Acetone. Results: There was a dose-dependent effect of the fruit extract on lifespan, fertility and oxidative stress resistance, with a unique information on the anti-ageing bioactivity of Ananas sativa. The IC 50 values of the fruit extract and Ascorbic acid positive control in the free radical scavenging activity were 248.15µg/ml and 81.51µg/ml respectively. However, the bioactive fraction obtained from the Ethylacetate fraction F1, did not exhibit oxidative stress resistant effect in the model organism. Conclusion:The methanol fruit extract of Ananas sativa possesses anti-ageing bioactivity through oxidative stress resistance.