Seaweeds can play a vital role in plant growth promotion. Two concentrations (5 and 10 mg/mL) of soluble polysaccharides extracted from the green macroalgae Ulva fasciata and Ulva lactuca were tested on Zea mays L. The carbohydrate and protein contents, and antioxidant activities (phenols, ascorbic, peroxidase, and catalase) were measured, as well as the protein banding patterns. The soluble polysaccharides at 5 mg/mL had the greatest effect on the base of all of the parameters. The highest effects of soluble polysaccharides on the Zea mays were 38.453, 96.76, 4, 835, 1.658, 7.462, and 38615.19, mg/mL for carbohydrates, proteins, phenol, µg ascorbic/mL, mg peroxidase/g dry tissue, and units/g tissue of catalase, respectively. The total number of protein bands (as determined by SDS PAGE) was not changed, but the density of the bands was correlated to the treatments. The highest band density and promoting effect were correlated to 5 mg/mL soluble polysaccharide treatments extracted from Ulva fasciata in Zea mays, which can be used as a biofertilizer.

Arbuscular mycorrhizal fungi (AMF) form a symbiotic relationship with plants; a symbiotic relationship is one in which both partners benefit from each other. Fungi benefit plants by improving uptake of water and nutrients, especially phosphorous, while plants provide 10–20% of their photosynthates to fungus. AMF tend to make associations with 85% of plant families and play a significant role in the sustainability of an ecosystem. Plants’ growth and productivity are negatively affected by various biotic and abiotic stresses. AMF proved to enhance plants’ tolerance against various stresses, such as drought, salinity, high temperature, and heavy metals. There are some obstacles impeding the beneficial formation of AMF communities, such as heavy tillage practices, high fertilizer rates, unchecked pesticide application, and monocultures. Keeping in view the stress-extenuation potential of AMF, the present review sheds light on their role in reducing erosion, nutrient leaching, and tolerance to abiotic stresses. In addition, recent advances in commercial production of AMF are discussed.

Methicillin-resistant Staphylococcus aureus (MRSA) causes life-threatening infections. Zinc oxide is well known as an effective antibacterial drug against many bacterial strains. We investigated the performance of zinc oxide nanorods synthesized by Albmiun as a biotemplate as an antibacterial drug in this study; the fabrication of zinc oxide nanorods was synthesized by sol–gel methods. We performed physicochemical characterization of zinc oxide nanorods by physiochemical techniques such as FTIR spectroscopy, X-ray diffraction, and TEM and investigation of their antimicrobial toxicity efficiency by MIC, ATPase activity assay, anti-biofilm activity, and kill time assays, as well as the mecA, mecR1, blaR1, blaZ, and biofilm genes (ica A, ica D, and fnb A) by using a quantitative RT-PCR assay and the penicillin-binding protein 2a (PBP2a) level of MRSA by using a Western blot. The data confirmed the fabrication of rod-shaped zinc oxide nanorods with a diameter in the range of 50 nm, which emphasized the formation of zinc oxide nanoparticles with regular shapes. The results show that zinc oxide nanorods inhibited methicillin-resistant S. aureus effectively. The MIC value was 23 μg/mL. The time kill of ZnO-NRs against MRSA was achieved after 2 h of incubation at 4MIC (92 μg/mL) and after 3 h of incubation at 2MIC (46 μg/mL), respectively. The lowest concentration of zinc oxide nanorods with over 75% biofilm killing in all strains tested was 32 μg/mL. Also, we examined the influence of the zinc oxide nanorods on MRSA by analyzing mecA, mecR1, blaR1, and blaZ by using a quantitative RT-PCR assay. The data obtained revealed that the presence of 2× MIC (46 μg/mL) of ZnO-NRs reduced the transcriptional levels of blaZ, blaR1, mecA, and mecR1 by 3.4-fold, 3.6-fold, 4-fold, and 3.8-fold, respectively. Furthermore, the gene expression of biofilm encoding genes (ica A, ica B, ica D, and fnb A) was tested using quantitative real-time reverse transcriptase-polymerase chain reaction (rt-PCR). The results showed that the presence of 2× MIC (46 μg/mL) of ZnO-NRs reduced the transcriptional levels of ica A, ica B, ica D, and fnb A. Also, the PBP2a level was markedly reduced after treatment with ZnO-NRs.

In this study, we explored the capacity for two promising macrophytes, Typha domingensis and Typha elephantina, to be used for the surveillance of contamination by six metals, i.e., Cu, Fe, Mn, Ni, Pb, and Zn, in the mountainous area of Taif City in Saudi Arabia. Regression models were generated in order to forecast the metal concentrations within the plants’ organs, i.e., the leaves, flowers, peduncles, rhizomes, and roots. The sediment mean values for pH and the six metals varied amongst the sampling locations for the respective macrophytes, indicating that similar life forms fail to indicate equivalent concentrations. For instance, dissimilar concentrations of the metals under investigation were observed within the organs of the two rooted macrophytes. The research demonstrated that the segregation of metals is a regular event in all the investigated species in which the metal concentrations vary amongst the different plant constituent types. In the current study, T. domingensis and T. elephantina varied in their capacity to absorb specific metals; the bioaccumulation of metals was greater within T. domingensis. The relationships between the observed and model-estimated metal levels, in combination with high R2 and modest mean averaged errors, offered an appraisal of the goodness of fit of most of the generated models. The t-tests revealed no variations between the observed and model-estimated concentrations of the six metals under investigation within the organs of the two macrophytes, which emphasised the precision of the models. These models offer the ability to perform hazard appraisals within ecosystems and to determine the reference criteria for sediment metal concentration. Lastly, T. domingensis and T. elephantina exhibit the potential for bioaccumulation for the alleviation of contamination from metals.

The aim of this study was to investigate the effect of a variable supply of potassium to culture medium on physiological and anatomical parameters (histological sections at the third internode) in basil, Ocimum basilicum. Thirty-four-day-old plants grown on basic nutrient medium were divided into four batches and grown on media with varying doses of potassium: 0.375 mM, 0.250 mM, 0.125 mM and 0 mM K+. After 64 days of culture, a final harvest was performed. The results showed that root and shoot growth in basil was decreased with decreased K+ concentration. This restriction was associated with a reduction in root elongation and leaf expansion, which was coupled with a decrease in chlorophyll and carotenoid contents. The estimation of electrolyte leakage reveals that this parameter was increased by potassium deficiency. With respect to total polyphenol and flavonoid contents, only the third leaf-stage extracts exhibited a decrease under low-K+ conditions. However, variability in response of phenolic compounds was recorded depending on the organ and the K+ concentration in the medium. Stem cross sections of potassium-deficient basil plants revealed a decrease in the diameter of these organs, which can be attributed to a restriction of the extent of different tissue territories (cortex and medulla), as well as by a reduction in cell size. These effects were associated with a decrease in the number of conducting vessels and an increase in the number of woody fibers.