Background: Oral cancer is one of the most common type of head and neck cancer, with a 5 year survival rate of < 50%. One of the major problems of oral cancer include the late stage of disease at the time of diagnosis. Mirror image biopsy is a new technique that can be used for detection of early changes in the oral mucosa. Aim of the study: To histologically assess the reflect copy biopsy occupied from clinically usual observing mucosa at consistent contralateral  anatomical place to the main lesion in patients identified with tongue squamous cell carcinoma to notice any indication of arena alteration in oral mucosa. Materials and methods: Seven patients diagnosed with squamous cell carcinoma of the tongue underwent reflect copy biopsy from clinically usual observing mucosa at consistent contralateral anatomical place to the main lesion. The reflect copy biopsy example were exposed to histopathological check. Results: of the seven patients included, four were male and three were female, with an age range from 45 years to 64 years (median 54.5 years). One of the biopsies revealed severe dysplasia and six-revealed hyperplasia of the epithelium. Conclusion: Mirror image biopsy is a useful tool to detect the early field changes in oral mucosa in order to prevent further progression to oral cancer.