This paper aims to exploit the traffic parameters setting in adaptive traffic control. In this study, is known as Dynamic Timing Optimiser (DTO). DTO is an online algorithm, uses real-time optimisation in estimating cycle length according to fluctuations arrival flow registered from the detector. DTO cycle time estimation is also incorporated with preset parameters including saturation flow rate (s) and lost time (L). However, these traffic flow parameters commonly inputted as one deterministic value which adopted for the whole day. For example, presumed constant of saturation flow rate (s) do not accurately represent an actual oversaturated condition. The effects of employing inaccurate saturation flow rate (s) lead to the underestimation of cycle length. Therefore, a set of parameters value is applied and tested encompass of default value and adjusted value that implied a heaviest traffic condition through microscopic simulation. This resulted in outcomes of intersection performance in terms of intersection delay, travel time and throughput. According to simulation result, saturation flow rate (s) parameters show a great influence in cycle length optimisation compared to lost time (L) parameter. Employing a realistic saturation flow rate (s) while inputting parameters in DTO according to real traffic conditions contribute to a less intersection delay. In addition, the study revealed that a longer lost time (L) configured in the signal system, a longer cycle length generated by DTO algorithm. As predicted, high delay occurs during long cycle length yet benefited in allowing a higher throughput.